Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MJ615_RS03620 Genome accession   NZ_CP092648
Coordinates   755413..756213 (-) Length   266 a.a.
NCBI ID   WP_004145898.1    Uniprot ID   -
Organism   Klebsiella pneumoniae strain KP7728     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 750413..761213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ615_RS03600 dksA 750993..751448 (-) 456 WP_002888845.1 RNA polymerase-binding protein DksA -
  MJ615_RS03605 sfsA 751611..752327 (-) 717 WP_002888848.1 DNA/RNA nuclease SfsA -
  MJ615_RS03610 thpR 752327..752863 (-) 537 WP_004177389.1 RNA 2',3'-cyclic phosphodiesterase -
  MJ615_RS03615 hrpB 752936..755365 (+) 2430 WP_023302300.1 ATP-dependent helicase HrpB -
  MJ615_RS03620 pilD 755413..756213 (-) 801 WP_004145898.1 A24 family peptidase Machinery gene
  MJ615_RS03625 - 756210..756956 (-) 747 WP_004145897.1 type II secretion system protein N -
  MJ615_RS03630 - 756946..757431 (-) 486 WP_004145896.1 type II secretion system protein M -
  MJ615_RS03635 gspL 757424..758617 (-) 1194 WP_029498035.1 type II secretion system protein GspL -
  MJ615_RS03640 gspK 758604..759590 (-) 987 WP_004177385.1 type II secretion system minor pseudopilin GspK -
  MJ615_RS03645 gspJ 759587..760183 (-) 597 WP_023302298.1 type II secretion system minor pseudopilin GspJ -
  MJ615_RS03650 gspI 760180..760545 (-) 366 WP_002889025.1 type II secretion system minor pseudopilin GspI -
  MJ615_RS03655 gspH 760542..761057 (-) 516 WP_004191951.1 type II secretion system minor pseudopilin GspH -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29389.01 Da        Isoelectric Point: 8.8894

>NTDB_id=659552 MJ615_RS03620 WP_004145898.1 755413..756213(-) (pilD) [Klebsiella pneumoniae strain KP7728]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide


Download         Length: 801 bp        

>NTDB_id=659552 MJ615_RS03620 WP_004145898.1 755413..756213(-) (pilD) [Klebsiella pneumoniae strain KP7728]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-109)

Type IV leader peptidase family (Peptidase_A24)

(123-231)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

49.434

99.624

0.492
  pilD Vibrio campbellii strain DS40M4

46.182

100

0.477
  pilD Acinetobacter nosocomialis M2

46.008

98.872

0.455
  pilD Acinetobacter baumannii D1279779

45.247

98.872

0.447
  pilD Neisseria gonorrhoeae MS11

43.295

98.12

0.425