Detailed information NTG0659413

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MKK01_RS21985
• Genome accession: NZ_CP092632
• Coordinates: 4552291..4553091 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain ML.9ba2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4547291..4558091

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKK01_RS21950 (MKK01_21950)	gspH	4547444..4547959 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
MKK01_RS21955 (MKK01_21955)	gspI	4547956..4548321 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
MKK01_RS21960 (MKK01_21960)	gspJ	4548318..4548914 (+)	597	WP_039102857.1	type II secretion system minor pseudopilin GspJ	-
MKK01_RS21965 (MKK01_21965)	gspK	4548911..4549891 (+)	981	WP_117130872.1	type II secretion system minor pseudopilin GspK	-
MKK01_RS21970 (MKK01_21970)	gspL	4549884..4551080 (+)	1197	WP_117130873.1	type II secretion system protein GspL	-
MKK01_RS21975 (MKK01_21975)	-	4551073..4551558 (+)	486	WP_012968921.1	type II secretion system protein M	-
MKK01_RS21980 (MKK01_21980)	-	4551548..4552294 (+)	747	WP_023322077.1	type II secretion system protein N	-
MKK01_RS21985 (MKK01_21985)	pilD	4552291..4553091 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
MKK01_RS21990 (MKK01_21990)	hrpB	4553138..4555567 (-)	2430	WP_110216553.1	ATP-dependent helicase HrpB	-
MKK01_RS21995 (MKK01_21995)	thpR	4555640..4556176 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
MKK01_RS22000 (MKK01_22000)	sfsA	4556176..4556892 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
MKK01_RS22005 (MKK01_22005)	dksA	4557055..4557510 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=659413 MKK01_RS21985 WP_008807384.1 4552291..4553091(+) (pilD) [Klebsiella variicola subsp. variicola strain ML.9ba2]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=659413 MKK01_RS21985 WP_008807384.1 4552291..4553091(+) (pilD) [Klebsiella variicola subsp. variicola strain ML.9ba2]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCACTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425