Detailed information NTG0659172

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MKS89_RS02400
• Genome accession: NZ_CP092587
• Coordinates: 517849..518721 (-)
• Length: 290 a.a.
• NCBI ID: WP_072960110.1
• Uniprot ID: A0A1M5CLQ4
• Organism: Vibrio gazogenes strain PB1
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 512849..523721

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKS89_RS02360 (MKS89_02360)	-	513023..513772 (-)	750	WP_072960129.1	DNA-binding domain-containing protein	-
MKS89_RS02365 (MKS89_02365)	-	513756..514736 (-)	981	WP_077316282.1	DUF692 domain-containing protein	-
MKS89_RS02370 (MKS89_02370)	-	514750..515025 (-)	276	WP_072960114.1	DUF2282 domain-containing protein	-
MKS89_RS02375 (MKS89_02375)	-	515111..515308 (-)	198	WP_205409134.1	zf-HC2 domain-containing protein	-
MKS89_RS02380 (MKS89_02380)	-	515305..515931 (-)	627	WP_083571270.1	RNA polymerase factor sigma-70	-
MKS89_RS02385 (MKS89_02385)	yacG	516178..516381 (-)	204	WP_021020548.1	DNA gyrase inhibitor YacG	-
MKS89_RS02390 (MKS89_02390)	zapD	516466..517206 (-)	741	WP_021020549.1	cell division protein ZapD	-
MKS89_RS02395 (MKS89_02395)	coaE	517239..517847 (-)	609	WP_072960112.1	dephospho-CoA kinase	-
MKS89_RS02400 (MKS89_02400)	pilD	517849..518721 (-)	873	WP_072960110.1	A24 family peptidase	Machinery gene
MKS89_RS02405 (MKS89_02405)	-	518828..520054 (-)	1227	WP_072960108.1	type II secretion system F family protein	-
MKS89_RS02410 (MKS89_02410)	-	520032..521855 (-)	1824	WP_072960106.1	ATPase, T2SS/T4P/T4SS family	-
MKS89_RS02415 (MKS89_02415)	-	522116..522556 (-)	441	WP_072960104.1	pilin	-
MKS89_RS02425 (MKS89_02425)	-	522739..523353 (-)	615	WP_072960102.1	hypothetical protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32423.74 Da
• Isoelectric Point: 7.6576

>NTDB_id=659172 MKS89_RS02400 WP_072960110.1 517849..518721(-) (pilD) [Vibrio gazogenes strain PB1]
MDAFLIYPWLFPVFASLFGLIIGSFLNVVIYRLPRMMVTNWKQEFAEAFPEYDIPSPTKKERISLSLPASFCPHCHHRIR
IWHNIPLFGWLLLGGKCADCGERISIQYPLVEFLTALASGIIAIHGGMTYYTLALFVFTYISIAAACIDLNQMLLPDNLT
LPLIWLGLLLSLTHINPLPLSDAVIGAVAGYLSLWSIYWMFKLLTKKEGMGYGDFKYLAALGAWIGWQTLPMVILLSSVI
GIILGVTLLLIRKQGNQTFPFGPSLAIAGWICLLWGDPMMSWYTSIVLGA

Nucleotide

• Length: 873 bp

>NTDB_id=659172 MKS89_RS02400 WP_072960110.1 517849..518721(-) (pilD) [Vibrio gazogenes strain PB1]
ATGGATGCATTTTTGATTTATCCTTGGTTATTTCCAGTTTTTGCGAGCCTCTTCGGCCTTATCATCGGCAGTTTTCTCAA
TGTCGTCATCTATCGTTTACCACGAATGATGGTCACAAACTGGAAGCAAGAATTTGCCGAAGCTTTCCCCGAGTACGATA
TTCCTTCACCAACGAAGAAGGAACGGATTTCCCTCAGTCTCCCGGCATCATTCTGTCCGCACTGCCATCACCGAATCCGG
ATTTGGCATAATATTCCACTGTTTGGCTGGTTATTACTCGGTGGCAAATGTGCAGATTGTGGTGAGCGTATATCGATACA
ATATCCGCTGGTAGAATTCTTGACGGCGCTGGCAAGCGGCATCATCGCGATTCATGGCGGTATGACTTATTACACGCTGG
CATTGTTTGTCTTTACTTACATCAGCATTGCCGCAGCATGTATCGATCTCAATCAGATGTTACTCCCTGATAATTTAACG
CTCCCGTTGATCTGGCTTGGGCTATTACTATCACTGACACACATCAATCCTCTACCTTTATCCGATGCGGTGATAGGCGC
GGTTGCCGGCTATTTATCTCTCTGGTCGATTTACTGGATGTTTAAACTCCTGACCAAAAAAGAAGGCATGGGATACGGTG
ACTTTAAATATCTTGCAGCCTTAGGGGCATGGATCGGCTGGCAGACGTTACCAATGGTGATTTTATTGTCCTCAGTGATT
GGCATCATTTTAGGTGTCACACTACTGTTGATAAGAAAACAAGGTAATCAGACCTTTCCATTCGGCCCTTCTCTGGCAAT
TGCCGGATGGATTTGTCTCCTCTGGGGCGACCCCATGATGAGCTGGTATACATCAATCGTGTTAGGAGCGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-125)

• Type IV leader peptidase family (Peptidase_A24) (137-246)

3D structure

• AlphaFold DB: A0A1M5CLQ4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	60	100	0.6
pilD	Vibrio cholerae strain A1552	59.31	100	0.593
pilD	Acinetobacter nosocomialis M2	49.632	93.793	0.466
pilD	Acinetobacter baumannii D1279779	48.529	93.793	0.455
pilD	Neisseria gonorrhoeae MS11	44.404	95.517	0.424