Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   MKI50_RS08035 Genome accession   NZ_CP092567
Coordinates   1653812..1656013 (-) Length   733 a.a.
NCBI ID   WP_001557362.1    Uniprot ID   A0A6K1MRM3
Organism   Staphylococcus aureus strain MRSA-WC000     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1648812..1661013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKI50_RS08020 (MKI50_001585) lepA 1650316..1652139 (-) 1824 WP_000368339.1 translation elongation factor 4 -
  MKI50_RS08025 (MKI50_001586) rpsT 1652485..1652736 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  MKI50_RS08030 (MKI50_001587) holA 1652781..1653755 (-) 975 WP_001282563.1 DNA polymerase III subunit delta -
  MKI50_RS08035 (MKI50_001588) comEC 1653812..1656013 (-) 2202 WP_001557362.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MKI50_RS08040 (MKI50_001589) - 1656018..1656479 (-) 462 WP_000439693.1 ComE operon protein 2 -
  MKI50_RS08045 (MKI50_001590) comEA 1656571..1657257 (-) 687 WP_001802022.1 ComEA family DNA-binding protein Machinery gene
  MKI50_RS08050 (MKI50_001591) - 1657297..1658013 (-) 717 WP_000084830.1 class I SAM-dependent methyltransferase -
  MKI50_RS08055 (MKI50_001592) rsfS 1658016..1658369 (-) 354 WP_001088020.1 ribosome silencing factor -
  MKI50_RS08060 (MKI50_001593) yqeK 1658370..1658954 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MKI50_RS08065 (MKI50_001594) nadD 1658944..1659513 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  MKI50_RS08070 (MKI50_001595) yhbY 1659516..1659806 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  MKI50_RS08075 (MKI50_001596) aroE 1659810..1660616 (-) 807 WP_000666757.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 733 a.a.        Molecular weight: 84998.05 Da        Isoelectric Point: 10.0028

>NTDB_id=658996 MKI50_RS08035 WP_001557362.1 1653812..1656013(-) (comEC) [Staphylococcus aureus strain MRSA-WC000]
MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPISLFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQ
VIQIQRQGSDTYKGRLSLKNEIYPFFLTDKKNFDLKKIESRNCIVKGQFKVNENKFVTLKLQSIVVQSCLESNRSNLIEK
HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAIVFLIYQPLKRLNLPLFVIKGIT
IIVLTLYAQYTNYAPSAVRAIIMTTLVLLITKQIKIKGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQ
QLSKLQSLFIITFIAQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYLVDLSFNFH
DWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKRKYILVTFWTIIILTLLITFPTNSHHKITMLNVGQGDSI
LYEGGKNQNVLIDTGGKVIDDTKQPSYSISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKG
YSSNTLMLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKKVLLMGDASKNNESLLL
KKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNL
KVDSNSYGNASGL

Nucleotide


Download         Length: 2202 bp        

>NTDB_id=658996 MKI50_RS08035 WP_001557362.1 1653812..1656013(-) (comEC) [Staphylococcus aureus strain MRSA-WC000]
TTGCTGTATGTCGCGTTATCAATGATTGTAGGAGTGCTTTGGAATTCTAGCAAAGTGCTCTCTACATTTCTTTTCATTTT
ACTTTTGTATATTACTTATCGTAAAAATAAAATCGTTTATGCCCCTATTTCTCTCTTTTTAATCATTTTCTCCTCATGGT
ATTTACATTATTCACAACAAGCAATATTTAATTATATCAATTATATTGAACGTAATTCTCAGTTTAATGAGCGTGCTCAA
GTAATCCAAATTCAACGTCAAGGTAGTGACACATATAAAGGTAGGTTGAGTTTAAAAAATGAAATATATCCTTTCTTTTT
AACAGATAAAAAGAATTTTGATTTAAAGAAAATTGAAAGTCGTAATTGTATTGTTAAAGGACAATTCAAAGTTAATGAAA
ATAAGTTTGTAACTCTTAAATTACAAAGTATAGTTGTACAAAGCTGCCTAGAATCGAACCGGTCTAATTTAATTGAGAAA
CATAAACAGTTTATAATGAATCGAATTTATGATTCGGGTATTAAGTTTCCGGATCGTATTATGGCATTGATTACTGGTGA
CGTAAAAGAAATTAATGAGCAATTTAAGGAACGTGTTAAAGAGATAGGTATATATCATTTGCTGGCAGTTAGTGGCTCGC
ATATAGCTGCAATTGTATTCTTAATTTACCAACCTTTAAAACGATTAAATTTACCTTTATTTGTCATTAAAGGAATTACA
ATCATTGTATTAACTTTATATGCTCAATACACAAATTATGCACCTAGTGCTGTAAGAGCTATAATAATGACAACTCTTGT
ACTGCTTATTACTAAGCAAATTAAAATAAAGGGTATTCAGCTATTAGCATTTGCATTTATAATTATGTTTATTTTAAACC
CACTAGTTGTTTATGATATTGGATTTCAATTTTCATTCATCATTTCATTTTTTATTATGCTACTTTTTCCTTTTTTACAG
CAATTGTCAAAGTTACAATCATTATTCATAATTACGTTTATTGCACAATTAGCTTCATTTATCGTTGCCATTCCAAACTT
TCATCAACTTCAATGGGTGGGATTTTTATCTAATTTGATTTTTGTACCGTACTATTCGATTATATTGTTTCCGCTATCTA
TTTTATTCTTTATTACAAGTCATTTTATTGTGGGATTAACGCCGCTAAATTACTTGGTTGACCTAAGTTTTAATTTTCAT
GACTGGTTACTAGACCTATTCACAAGAATTAAGCAATCACATTTTTCTGTTCCCAAGTTTAATGATTGGATATTTATAAT
ATTTATAATTTCTGTTTATTACATATTTTGGTTATTGGCTAAACGTAAATATATATTGGTTACGTTTTGGACTATAATTA
TTCTGACATTATTAATAACGTTTCCAACAAATTCACATCACAAAATTACAATGTTAAATGTGGGGCAGGGAGACAGTATT
TTATATGAAGGCGGTAAGAACCAAAATGTCTTGATTGATACAGGTGGGAAAGTGATTGATGATACTAAACAACCTAGTTA
TTCAATTTCTAAATATCATATTTTACCAACGCTAAATGAAAGAGGGATAAATGAATTAGAGTATCTAATTTTAACACATC
CACACAATGACCATATTGGTGAAGTGGAATATATTATTAGTCATATTAAAATTAAACATATAGTGATATACAATAAGGGA
TATAGTAGTAATACATTGATGTTATTATCGAAATTAAGCCATAAGTACAACATTAAACTTATGGATGTAAGACAAGTTAG
TAGTTTTAAACTTGGAGATAGTAGTTTTCTATTTTTTGATAGTTTTATTCCAAATAGCCGAGATAAAAATGAGTATTCGA
TTATTACTATGATTACATATCAAAATAAAAAAGTTTTATTAATGGGCGATGCTAGTAAAAATAATGAATCTTTACTACTA
AAAAAATATAACTTGCCGGAGATTGATATTTTAAAAGTAGGACATCATGGGAGCAAGACAAGTAGTTCTAAAGAATTTAT
AGAGATGATTAAGCCTAAAATAAGTTTGATTTCTTCTGGGAAGAACAATATGTATCATCTTCCTAATATAGAAGTTGTTA
AACGATTGCAAAGGATTCGCAGTCGCATTTACAATAGTCAACAAAACGGTCAAGTTACAATTGACTTAGATGATAATTTA
AAAGTTGATTCAAACTCTTATGGAAATGCAAGTGGTTTATAG

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(474-679)

Competence protein (Competence)

(183-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6K1MRM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Staphylococcus aureus N315

100

100

1
  comEC Staphylococcus aureus MW2

98.772

100

0.988