Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   MKI54_RS07470 Genome accession   NZ_CP092561
Coordinates   1566789..1567286 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain MRSA-WC101     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1561789..1572286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKI54_RS07445 (MKI54_001473) gcvPB 1561951..1563423 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  MKI54_RS07450 (MKI54_001474) gcvPA 1563416..1564762 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  MKI54_RS07455 (MKI54_001475) gcvT 1564782..1565873 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  MKI54_RS07460 (MKI54_001476) - 1566032..1566556 (-) 525 WP_001015120.1 shikimate kinase -
  MKI54_RS07465 (MKI54_001477) - 1566546..1566692 (-) 147 WP_001792109.1 hypothetical protein -
  MKI54_RS07470 (MKI54_001478) comGF 1566789..1567286 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  MKI54_RS07475 (MKI54_001479) comGE 1567204..1567503 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  MKI54_RS07480 (MKI54_001480) comGD 1567490..1567936 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  MKI54_RS07485 (MKI54_001481) comGC 1567914..1568225 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  MKI54_RS07490 (MKI54_001482) comGB 1568239..1569309 (-) 1071 WP_000776424.1 competence type IV pilus assembly protein ComGB Machinery gene
  MKI54_RS07495 (MKI54_001483) comGA 1569281..1570255 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  MKI54_RS07500 (MKI54_001484) - 1570307..1570930 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  MKI54_RS07505 (MKI54_001485) - 1570927..1571256 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  MKI54_RS07510 (MKI54_001486) - 1571256..1572242 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=658858 MKI54_RS07470 WP_001788897.1 1566789..1567286(-) (comGF) [Staphylococcus aureus strain MRSA-WC101]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=658858 MKI54_RS07470 WP_001788897.1 1566789..1567286(-) (comGF) [Staphylococcus aureus strain MRSA-WC101]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964