Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   MKI60_RS07475 Genome accession   NZ_CP092544
Coordinates   1567700..1568197 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain VMRSA-WC102     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1562700..1573197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKI60_RS07450 (MKI60_001475) gcvPB 1562862..1564334 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  MKI60_RS07455 (MKI60_001476) gcvPA 1564327..1565673 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  MKI60_RS07460 (MKI60_001477) gcvT 1565693..1566784 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  MKI60_RS07465 (MKI60_001478) - 1566943..1567467 (-) 525 WP_001015120.1 shikimate kinase -
  MKI60_RS07470 (MKI60_001479) - 1567457..1567603 (-) 147 WP_001792109.1 hypothetical protein -
  MKI60_RS07475 (MKI60_001480) comGF 1567700..1568197 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  MKI60_RS07480 (MKI60_001481) comGE 1568115..1568414 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  MKI60_RS07485 (MKI60_001482) comGD 1568401..1568847 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  MKI60_RS07490 (MKI60_001483) comGC 1568825..1569136 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  MKI60_RS07495 (MKI60_001484) comGB 1569150..1570220 (-) 1071 WP_000776424.1 competence type IV pilus assembly protein ComGB Machinery gene
  MKI60_RS07500 (MKI60_001485) comGA 1570192..1571166 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  MKI60_RS07505 (MKI60_001486) - 1571218..1571841 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  MKI60_RS07510 (MKI60_001487) - 1571838..1572167 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  MKI60_RS07515 (MKI60_001488) - 1572167..1573153 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=658554 MKI60_RS07475 WP_001788897.1 1567700..1568197(-) (comGF) [Staphylococcus aureus strain VMRSA-WC102]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=658554 MKI60_RS07475 WP_001788897.1 1567700..1568197(-) (comGF) [Staphylococcus aureus strain VMRSA-WC102]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964