Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MS6_RS12285 Genome accession   NZ_AP014524
Coordinates   2664856..2665872 (-) Length   338 a.a.
NCBI ID   WP_001898302.1    Uniprot ID   -
Organism   Vibrio cholerae MS6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2659856..2670872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS6_RS12260 (MS6_2350) aroK 2660739..2661263 (-) 525 WP_000818616.1 shikimate kinase AroK -
  MS6_RS12265 (MS6_2351) pilQ 2661450..2663165 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  MS6_RS12270 (MS6_2352) pilP 2663191..2663709 (-) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  MS6_RS12275 (MS6_2353) pilO 2663699..2664289 (-) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  MS6_RS12280 (MS6_2354) pilN 2664282..2664866 (-) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  MS6_RS12285 (MS6_2355) pilM 2664856..2665872 (-) 1017 WP_001898302.1 type IV pilus assembly protein PilM Machinery gene
  MS6_RS12290 (MS6_2356) - 2666015..2668492 (+) 2478 WP_032481152.1 penicillin-binding protein 1A -
  MS6_RS12295 (MS6_2357) oxyR 2668565..2669458 (-) 894 WP_001914100.1 DNA-binding transcriptional regulator OxyR -
  MS6_RS12300 (MS6_2358) - 2669598..2670329 (+) 732 WP_000495223.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37735.49 Da        Isoelectric Point: 7.3328

>NTDB_id=65835 MS6_RS12285 WP_001898302.1 2664856..2665872(-) (pilM) [Vibrio cholerae MS6]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLVRSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=65835 MS6_RS12285 WP_001898302.1 2664856..2665872(-) (pilM) [Vibrio cholerae MS6]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGTCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTACCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCAGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.704

100

0.997
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment