Detailed information NTG0658287

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MKM89_RS21285
• Genome accession: NZ_CP092512
• Coordinates: 4340717..4341517 (+)
• Length: 266 a.a.
• NCBI ID: WP_049093658.1
• Uniprot ID: -
• Organism: Klebsiella quasipneumoniae strain EF262
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4335717..4346517

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKM89_RS21250 (MKM89_21250)	gspH	4335870..4336385 (+)	516	WP_004204416.1	type II secretion system minor pseudopilin GspH	-
MKM89_RS21255 (MKM89_21255)	gspI	4336382..4336747 (+)	366	WP_004204415.1	type II secretion system minor pseudopilin GspI	-
MKM89_RS21260 (MKM89_21260)	gspJ	4336744..4337340 (+)	597	WP_065904245.1	type II secretion system minor pseudopilin GspJ	-
MKM89_RS21265 (MKM89_21265)	gspK	4337337..4338317 (+)	981	WP_004204412.1	type II secretion system minor pseudopilin GspK	-
MKM89_RS21270 (MKM89_21270)	gspL	4338310..4339506 (+)	1197	WP_004204411.1	type II secretion system protein GspL	-
MKM89_RS21275 (MKM89_21275)	-	4339499..4339984 (+)	486	WP_004204409.1	type II secretion system protein M	-
MKM89_RS21280 (MKM89_21280)	-	4339974..4340702 (+)	729	WP_048294185.1	type II secretion system protein N	-
MKM89_RS21285 (MKM89_21285)	pilD	4340717..4341517 (+)	801	WP_049093658.1	A24 family peptidase	Machinery gene
MKM89_RS21290 (MKM89_21290)	hrpB	4341565..4343994 (-)	2430	WP_153245303.1	ATP-dependent helicase HrpB	-
MKM89_RS21295 (MKM89_21295)	thpR	4344067..4344603 (+)	537	WP_004204405.1	RNA 2',3'-cyclic phosphodiesterase	-
MKM89_RS21300 (MKM89_21300)	sfsA	4344603..4345319 (+)	717	WP_049093657.1	DNA/RNA nuclease SfsA	-
MKM89_RS21305 (MKM89_21305)	dksA	4345482..4345937 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29382.98 Da
• Isoelectric Point: 8.8894

>NTDB_id=658287 MKM89_RS21285 WP_049093658.1 4340717..4341517(+) (pilD) [Klebsiella quasipneumoniae strain EF262]
MTTLAALSLHFPLIWYGFLLLFGLALGSFYNVVIYRLPRMLAQTADDEPLTLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQARISWSYPLTELTTGLLFILAGALLAPGLALAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQALPQVLLFASASGLMWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=658287 MKM89_RS21285 WP_049093658.1 4340717..4341517(+) (pilD) [Klebsiella quasipneumoniae strain EF262]
ATGACGACATTAGCCGCTTTATCGCTGCATTTCCCTCTTATCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCCCGCATGCTGGCCCAGACCGCGGACGACGAGCCGCTAACCCTCAGCA
CGCCGGGCTCATCCTGTCCGCAGTGTCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGCTTCCTCTGGCTA
GGCCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGACCACCGGCCTGCTGTTTAT
CCTCGCGGGGGCGCTGCTCGCACCGGGACTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTTCTGCTGATACTGGCGC
GGATTGATGCCCGGACCCAGCTGCTGCCCGATCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTTAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCGATGGCTGGTTATCTGGCACTGTGGTCGGTCTATTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCTCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGCTCTGCCGCAGGTGCTGTTGTTCGCCTCGGCCAGCGGGCTGATGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425