Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MIS46_RS00685 Genome accession   NZ_CP092358
Coordinates   120649..122031 (+) Length   460 a.a.
NCBI ID   WP_249442026.1    Uniprot ID   -
Organism   Wielerella bovis strain 09LB414     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 115649..127031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS46_RS00665 (MIS46_00665) dprA 115838..117040 (+) 1203 WP_249442781.1 DNA-processing protein DprA Machinery gene
  MIS46_RS00670 (MIS46_00670) tsf 117443..118297 (-) 855 WP_249442782.1 translation elongation factor Ts -
  MIS46_RS00675 (MIS46_00675) rpsB 118382..119110 (-) 729 WP_249442783.1 30S ribosomal protein S2 -
  MIS46_RS00680 (MIS46_00680) - 119383..120561 (+) 1179 WP_249442784.1 phosphoglycerate kinase -
  MIS46_RS00685 (MIS46_00685) radA 120649..122031 (+) 1383 WP_249442026.1 DNA repair protein RadA Machinery gene
  MIS46_RS00690 (MIS46_00690) - 122161..122580 (+) 420 WP_249442785.1 SMI1/KNR4 family protein -
  MIS46_RS00695 (MIS46_00695) - 122637..123656 (-) 1020 WP_249442786.1 IS110 family transposase -
  MIS46_RS00700 (MIS46_00700) - 124061..124327 (+) 267 Protein_133 transposase -
  MIS46_RS00705 (MIS46_00705) - 124302..124844 (-) 543 WP_283397300.1 IS630 family transposase -
  MIS46_RS00710 (MIS46_00710) - 124863..125138 (-) 276 WP_249442787.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49530.20 Da        Isoelectric Point: 6.6883

>NTDB_id=656688 MIS46_RS00685 WP_249442026.1 120649..122031(+) (radA) [Wielerella bovis strain 09LB414]
MAKAPKTLYQCTECGGTSPKWVGKCPHCGEWNTLQENIAAPEPKNARFQSWAAETTQVQALSQVTAMEVPREATGMGELD
RVLGGGLVDGAVILLGGDPGIGKSTLLLQTIALMAKTRNVLYISGEESAQQVALRAQRLGLNAEGVNLLAEIRLEAIQAA
LKQHAPSVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRIAKQMGIAMIFVGHVTKDGAIAGPRVLEHMVDTVLYFEG
DQHSNHRMIRAIKNRFGAANELGVFAMTETGLKGVSNPSAIFLASYRDDVAGSCVLVTQEGTRPLLVEIQSLVDDAHGFT
PKRLTVGLEQNRLAMLLAVLNRHAGIACFDQDVFLNAVGGVKIGEPAADLAAVLAMVSSFRNKPLPEKMVAFGEIGLSGE
IRPVPRGQERLKEAEKLGFKRAIVPKANMPRNENEFPKLKIYGVESLQEAVMLCRDLVDE

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=656688 MIS46_RS00685 WP_249442026.1 120649..122031(+) (radA) [Wielerella bovis strain 09LB414]
ATGGCAAAAGCCCCCAAAACCCTCTACCAATGCACCGAATGCGGCGGCACATCGCCCAAATGGGTCGGCAAATGCCCCCA
TTGTGGCGAATGGAACACGCTACAAGAAAACATCGCGGCCCCCGAACCCAAAAACGCCCGTTTCCAATCATGGGCAGCCG
AAACGACACAAGTCCAAGCCCTATCCCAAGTTACCGCCATGGAAGTGCCACGCGAAGCCACAGGCATGGGCGAATTAGAC
CGCGTTTTGGGTGGCGGTTTGGTGGACGGAGCGGTAATTTTATTGGGTGGCGACCCTGGCATTGGCAAATCCACCTTATT
GTTACAAACCATTGCTTTAATGGCGAAAACGCGCAATGTTTTGTACATTTCAGGCGAAGAATCTGCGCAACAAGTCGCCC
TACGCGCCCAACGTTTGGGCTTGAATGCGGAAGGCGTGAATTTGCTTGCCGAAATCCGTTTGGAAGCCATTCAGGCAGCC
TTAAAGCAACACGCGCCCAGCGTGGTCGTGATTGACTCCATTCAAACCATGTATTCCGACCAAATCACGTCTGCCCCAGG
TTCCGTGTCGCAAGTGCGCGAATGTGCCGCCCAACTCACGCGCATCGCCAAACAAATGGGCATCGCCATGATTTTTGTCG
GACACGTTACCAAAGACGGTGCGATTGCGGGCCCGCGCGTGTTGGAACACATGGTGGACACCGTGTTGTATTTTGAAGGC
GACCAGCATTCCAATCATCGCATGATACGCGCGATTAAAAACCGCTTTGGCGCGGCAAACGAATTGGGCGTGTTCGCCAT
GACCGAAACGGGTTTGAAAGGCGTGTCCAATCCGTCCGCGATTTTTTTGGCGAGTTATCGGGACGATGTGGCGGGTTCGT
GCGTGTTGGTAACGCAGGAAGGGACGCGCCCATTATTAGTGGAAATTCAATCGCTTGTAGATGACGCACACGGTTTCACG
CCTAAACGCTTAACGGTCGGTTTGGAGCAAAACCGTTTGGCGATGTTGCTGGCGGTGCTGAACCGTCATGCGGGCATTGC
CTGTTTTGACCAAGACGTGTTTTTGAACGCGGTGGGCGGCGTGAAAATTGGCGAACCTGCGGCGGATTTGGCGGCGGTGT
TGGCGATGGTGTCCAGTTTCAGAAACAAGCCGCTGCCTGAAAAAATGGTGGCGTTTGGCGAAATCGGTTTGAGTGGCGAA
ATCCGCCCCGTGCCGCGCGGTCAGGAGCGTTTGAAAGAGGCGGAAAAATTGGGTTTCAAACGCGCCATTGTGCCGAAAGC
GAATATGCCGCGCAATGAAAACGAGTTCCCCAAATTAAAAATTTATGGCGTGGAAAGTTTGCAAGAAGCGGTTATGTTGT
GTCGGGATTTAGTTGATGAATGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(366-428)

AAA domain (AAA_25)

(80-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.096

99.13

0.507
  radA Streptococcus pneumoniae Rx1

48.009

98.261

0.472
  radA Streptococcus pneumoniae D39

48.009

98.261

0.472
  radA Streptococcus pneumoniae R6

48.009

98.261

0.472
  radA Streptococcus pneumoniae TIGR4

48.009

98.261

0.472
  radA Streptococcus mitis SK321

47.692

98.913

0.472
  radA Streptococcus mitis NCTC 12261

47.473

98.913

0.47