Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MIS44_RS06410 Genome accession   NZ_CP092357
Coordinates   1249695..1250876 (+) Length   393 a.a.
NCBI ID   WP_283397303.1    Uniprot ID   -
Organism   Wielerella bovis strain 10LB456     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1244695..1255876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS44_RS06380 (MIS44_06380) - 1245327..1246121 (-) 795 WP_249445191.1 c-type cytochrome -
  MIS44_RS06390 (MIS44_06390) - 1246821..1247189 (+) 369 WP_249445190.1 hemerythrin domain-containing protein -
  MIS44_RS06395 (MIS44_06395) - 1247384..1247818 (+) 435 WP_249447911.1 META domain-containing protein -
  MIS44_RS06400 (MIS44_06400) folP 1247839..1248684 (+) 846 WP_249447912.1 dihydropteroate synthase -
  MIS44_RS06405 (MIS44_06405) - 1248752..1249522 (+) 771 WP_249447913.1 ADP-ribosylglycohydrolase family protein -
  MIS44_RS06410 (MIS44_06410) pilU 1249695..1250876 (+) 1182 WP_283397303.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MIS44_RS06415 (MIS44_06415) - 1251297..1251983 (-) 687 WP_249447914.1 sulfite exporter TauE/SafE family protein -
  MIS44_RS06420 (MIS44_06420) - 1252215..1252823 (+) 609 WP_249447915.1 nitroreductase family protein -
  MIS44_RS06425 (MIS44_06425) - 1252953..1254839 (+) 1887 WP_249447916.1 lytic transglycosylase domain-containing protein -
  MIS44_RS06430 (MIS44_06430) grxB 1254914..1255564 (+) 651 WP_249447917.1 glutaredoxin 2 -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 43860.29 Da        Isoelectric Point: 5.6463

>NTDB_id=656675 MIS44_RS06410 WP_283397303.1 1249695..1250876(+) (pilU) [Wielerella bovis strain 10LB456]
MSALPPVNEEMKKFIHSLLTHMVKNKGSDLFITAGFPPAMKLDGKLTRLSDKPLTAEHTMQIARSIMDDKQAEEFLNTNE
CNFAISLAGVSRFRINAMIQRGAAALVCRVITSDIPKFEDLKLPPVLRDVVMEKRGLVIFVGGTGSGKSTSLAAMIDYRN
ENSFGHIITIEDPIEYVHPHKNCIVSQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMATL
HANNSNQALDRIINFFPEERRTQLLTDLSLNLKGFISQRLIPRKTGKGRSAAVEILLNSPLISELILKGEVHGIKEIMAK
SRELGMQTFDQALFDLYEDDMISYDDAIKNADSANDLRLQIKLNSKKNTTAGEDGALDGLSIMDYQHEQPDEQ

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=656675 MIS44_RS06410 WP_283397303.1 1249695..1250876(+) (pilU) [Wielerella bovis strain 10LB456]
ATGTCCGCACTCCCACCCGTAAACGAAGAAATGAAAAAGTTCATTCACAGCTTATTGACTCACATGGTCAAAAACAAAGG
CTCGGACTTATTCATCACCGCAGGTTTCCCCCCCGCAATGAAATTGGACGGCAAATTAACCCGTTTGAGCGATAAACCGC
TGACTGCCGAACATACTATGCAAATTGCACGTTCCATTATGGACGACAAACAAGCCGAAGAATTTTTAAACACCAACGAA
TGCAACTTCGCTATCAGTTTGGCAGGCGTATCCCGTTTCCGTATTAACGCCATGATTCAACGTGGTGCAGCCGCTTTGGT
TTGCCGTGTGATTACCAGCGATATTCCCAAATTTGAAGATTTAAAATTGCCGCCTGTGTTGCGTGATGTGGTTATGGAAA
AACGCGGTTTAGTCATTTTTGTTGGTGGTACAGGTTCGGGTAAATCCACATCTCTCGCCGCTATGATTGATTATCGCAAT
GAAAACAGCTTTGGTCATATCATTACCATTGAAGACCCGATTGAATATGTGCATCCACACAAAAATTGTATCGTTTCACA
ACGCGAAGTTGGCGTTGATACCGAAAACTGGTTTGCCGCATTGAAAAACACCTTGCGCCAAGCCCCAGATGTGATTTTGA
TTGGTGAGATTCGCGACCGCGAAACCATGGACTACGCGCTTGCTTTTGCCGAAACAGGACACTTGTGTATGGCAACCTTG
CATGCCAACAACTCTAACCAAGCACTTGACCGCATCATCAACTTCTTCCCCGAAGAACGCCGCACCCAATTGCTGACTGA
CTTATCTTTGAACTTAAAAGGTTTCATTTCGCAACGTTTGATTCCACGAAAAACAGGTAAAGGTCGTTCAGCAGCAGTAG
AAATTTTGTTGAACTCGCCTTTGATTTCCGAATTGATTTTGAAAGGCGAAGTGCATGGCATTAAAGAAATCATGGCGAAA
TCACGCGAATTGGGCATGCAAACTTTTGACCAAGCCTTGTTTGATTTGTATGAAGACGACATGATTTCCTATGACGATGC
AATTAAAAATGCCGACTCCGCCAATGACTTGCGTTTACAAATTAAATTGAACAGTAAGAAAAATACCACAGCTGGCGAAG
ATGGTGCATTGGACGGCTTGTCTATTATGGATTATCAGCACGAACAACCTGATGAACAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.969

89.313

0.545
  pilU Acinetobacter baylyi ADP1

59.659

89.567

0.534
  pilU Vibrio cholerae strain A1552

55.096

92.366

0.509
  pilT Legionella pneumophila strain Lp02

43.243

84.733

0.366
  pilT Legionella pneumophila strain ERS1305867

43.243

84.733

0.366
  pilT Pseudomonas aeruginosa PAK

42.433

85.751

0.364
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.771

84.478

0.361