Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MIS44_RS05275 Genome accession   NZ_CP092357
Coordinates   1012565..1014670 (+) Length   701 a.a.
NCBI ID   WP_249449424.1    Uniprot ID   -
Organism   Wielerella bovis strain 10LB456     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1007565..1019670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS44_RS05255 (MIS44_05255) pilM 1009602..1010714 (+) 1113 WP_249443534.1 type IV pilus assembly protein PilM -
  MIS44_RS05260 (MIS44_05260) - 1010716..1011333 (+) 618 WP_249443535.1 PilN domain-containing protein -
  MIS44_RS05265 (MIS44_05265) pilO 1011330..1011974 (+) 645 WP_249443536.1 type 4a pilus biogenesis protein PilO -
  MIS44_RS05270 (MIS44_05270) - 1012000..1012527 (+) 528 WP_249443537.1 pilus assembly protein PilP -
  MIS44_RS05275 (MIS44_05275) pilQ 1012565..1014670 (+) 2106 WP_249449424.1 type IV pilus secretin PilQ Machinery gene
  MIS44_RS05280 (MIS44_05280) - 1014723..1015235 (+) 513 WP_249449425.1 shikimate kinase -
  MIS44_RS05285 (MIS44_05285) aroB 1015245..1016327 (+) 1083 WP_249446507.1 3-dehydroquinate synthase -
  MIS44_RS05290 (MIS44_05290) - 1016357..1016776 (+) 420 WP_249449426.1 ProQ/FINO family protein -
  MIS44_RS05295 (MIS44_05295) - 1016895..1017464 (+) 570 WP_249449427.1 peptidylprolyl isomerase -
  MIS44_RS05300 (MIS44_05300) - 1017556..1018065 (+) 510 WP_249449428.1 peptidylprolyl isomerase -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 76427.49 Da        Isoelectric Point: 9.4091

>NTDB_id=656672 MIS44_RS05275 WP_249449424.1 1012565..1014670(+) (pilQ) [Wielerella bovis strain 10LB456]
MKINSIKIMSAVALGLLFQAANAGSITDINVSVLPNKQRVIKFKFDSGVVEPSGFITSAPARIALDFPATDIKVSQSQLT
FNDQLLKQIIAAQGSEHARVLLGLGKEGQYSTEIKGNEVWVYLSEAESTNTAAVAAAQSVVSSAPKNSQSYQSSGITAPF
NIDFHKGANRSGVIEYSSNYSGQPQMRVQSDRLIILLKDYPLSVQDQRNLDVTDFSTPVRTINVRRIGNDAQIIIRSQGT
WEHKVSGRNGRQTIHIVPTKNISTIGLSRKNDQKFTGKNVSLDFQNVDVRTVLQILATESGMNIVASDNVTGKMTLSLKD
VPWDQALDLILDARNLDMRKVGNIINIAPRSDLLARDTEALEAQRKLDDLGPLISQSFQLKYKNVEEFRKILRISDSTSV
SSDRNSILSSRGSALIDPATNTLIVTDVQSVITKFNKLIEELDVPSRQVMIEARIVEAADGFSRDLGVQFGYQRSGAHTS
VGSNWSNSINNANVGYGSATSSILNPNVALPSSASTSSIGIVRAVSSGALGLELSASESENRSKTISTPRVLTQDRQEAE
IRQGYQIPYTKREDGETSIEWKAAELVLKTTPRITPDNKVILDIEIKKDEPDGTYTAITGEAGLATRYVKTQAMIEDGGT
LVVGGIYQEATSNSIRKVPLLGDIPVVGNLFKARARKSSRNELLFFITPRIMGSETSVLRY

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=656672 MIS44_RS05275 WP_249449424.1 1012565..1014670(+) (pilQ) [Wielerella bovis strain 10LB456]
ATGAAAATAAATTCTATCAAAATAATGTCAGCAGTAGCATTAGGTTTATTATTTCAAGCTGCAAATGCGGGAAGTATTAC
TGATATTAATGTTTCGGTACTTCCAAATAAACAACGTGTAATTAAATTCAAGTTTGATAGTGGTGTGGTTGAACCTAGTG
GTTTTATTACATCAGCTCCTGCTCGTATTGCTTTGGATTTTCCTGCAACAGATATTAAAGTTTCACAATCTCAATTAACT
TTTAATGATCAACTGCTAAAACAGATTATTGCTGCACAAGGTAGTGAGCATGCTCGTGTACTGTTGGGGTTGGGTAAAGA
AGGACAATATAGTACTGAAATTAAAGGAAATGAGGTTTGGGTATATTTGAGTGAAGCTGAGTCAACTAATACAGCTGCAG
TTGCTGCTGCGCAATCTGTTGTAAGCTCGGCTCCTAAAAATTCACAATCTTACCAATCTTCTGGAATAACAGCGCCATTT
AATATTGATTTCCATAAAGGTGCAAATAGATCAGGTGTAATTGAGTATTCATCAAATTATTCAGGTCAGCCTCAAATGAG
AGTGCAAAGTGATCGATTGATTATCTTGTTAAAAGATTATCCATTGTCGGTTCAAGATCAACGTAATTTAGATGTAACCG
ATTTTTCTACTCCTGTGCGTACTATTAATGTTCGTCGTATTGGTAATGATGCGCAGATTATTATCAGAAGTCAGGGAACA
TGGGAGCATAAAGTTTCTGGTAGAAATGGACGCCAAACAATTCATATTGTTCCAACGAAAAATATTTCTACTATTGGGTT
AAGTCGGAAGAATGATCAGAAATTTACAGGAAAGAATGTTTCATTAGATTTCCAAAATGTTGATGTTCGTACTGTATTGC
AGATTCTAGCAACAGAGTCAGGAATGAATATTGTTGCGAGTGATAATGTAACAGGTAAAATGACATTGTCATTGAAAGAT
GTACCTTGGGATCAGGCTTTAGATTTGATTTTGGATGCACGCAATTTGGATATGCGTAAAGTTGGTAATATTATCAATAT
TGCACCGCGTTCAGATTTGTTGGCTCGTGATACAGAAGCACTTGAAGCTCAAAGAAAATTGGATGATTTAGGTCCTCTGA
TTTCTCAAAGTTTTCAATTGAAATATAAAAATGTTGAGGAATTTAGAAAAATTTTACGCATTAGTGATTCTACATCAGTA
AGTTCTGATAGAAATTCAATCCTTAGTTCACGTGGTAGTGCGTTGATTGATCCTGCAACAAATACATTGATCGTTACTGA
TGTCCAATCTGTAATTACAAAATTTAATAAATTAATTGAAGAATTGGATGTTCCTTCTCGTCAAGTAATGATTGAAGCAC
GTATTGTTGAAGCTGCTGATGGATTTTCGCGTGATTTAGGTGTTCAATTTGGTTATCAGCGTTCAGGAGCACATACTTCT
GTAGGCTCAAATTGGTCTAATAGTATTAATAATGCGAATGTTGGTTATGGGTCGGCTACATCAAGTATTTTAAATCCAAA
TGTTGCATTACCTTCTTCTGCATCAACAAGTTCTATCGGTATTGTGCGAGCTGTGTCGTCAGGTGCATTGGGTTTGGAAT
TGTCGGCATCAGAGTCGGAAAATCGCAGTAAAACGATTTCTACGCCACGCGTATTAACTCAAGATCGTCAAGAAGCGGAA
ATTCGTCAAGGTTATCAGATTCCTTATACAAAAAGGGAAGATGGTGAAACTTCTATCGAATGGAAAGCTGCGGAATTGGT
CTTGAAAACAACACCTCGTATTACACCAGATAACAAAGTTATTTTGGATATTGAAATTAAAAAAGATGAACCTGATGGTA
CTTATACCGCAATCACAGGTGAAGCAGGTCTTGCAACACGTTATGTGAAAACACAAGCAATGATTGAAGATGGTGGCACA
TTGGTTGTGGGTGGTATTTATCAAGAAGCAACATCTAATTCAATTCGTAAAGTACCTTTACTGGGTGATATTCCAGTTGT
TGGTAATTTATTTAAAGCGCGAGCGCGTAAAAGTTCACGCAATGAATTGCTGTTCTTCATTACACCGCGTATTATGGGTT
CTGAAACCAGCGTATTACGTTACTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

50.843

100

0.516
  pilQ Neisseria meningitidis M1080

48

100

0.514
  pilQ Neisseria meningitidis MC58

53.455

78.459

0.419