Detailed information NTG0656445

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MHK77_RS21155
• Genome accession: NZ_CP092278
• Coordinates: 4310171..4310971 (+)
• Length: 266 a.a.
• NCBI ID: WP_032419388.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain T877-PC
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4305171..4315971

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MHK77_RS21120 (MHK77_21090)	gspH	4305327..4305842 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
MHK77_RS21125 (MHK77_21095)	gspI	4305839..4306204 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
MHK77_RS21130 (MHK77_21100)	gspJ	4306201..4306797 (+)	597	WP_032419387.1	type II secretion system minor pseudopilin GspJ	-
MHK77_RS21135 (MHK77_21105)	gspK	4306794..4307780 (+)	987	WP_077259564.1	type II secretion system minor pseudopilin GspK	-
MHK77_RS21140 (MHK77_21110)	gspL	4307767..4308960 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
MHK77_RS21145 (MHK77_21115)	-	4308953..4309438 (+)	486	WP_004145896.1	type II secretion system protein M	-
MHK77_RS21150 (MHK77_21120)	-	4309428..4310174 (+)	747	WP_241511179.1	type II secretion system protein N	-
MHK77_RS21155 (MHK77_21125)	pilD	4310171..4310971 (+)	801	WP_032419388.1	A24 family peptidase	Machinery gene
MHK77_RS21160 (MHK77_21130)	hrpB	4311019..4313448 (-)	2430	WP_032419389.1	ATP-dependent helicase HrpB	-
MHK77_RS21165 (MHK77_21135)	thpR	4313521..4314057 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
MHK77_RS21170 (MHK77_21140)	sfsA	4314057..4314773 (+)	717	WP_032439619.1	DNA/RNA nuclease SfsA	-
MHK77_RS21175 (MHK77_21145)	dksA	4314936..4315391 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29477.07 Da
• Isoelectric Point: 8.6098

>NTDB_id=656445 MHK77_RS21155 WP_032419388.1 4310171..4310971(+) (pilD) [Klebsiella pneumoniae strain T877-PC]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTEDDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLTPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=656445 MHK77_RS21155 WP_032419388.1 4310171..4310971(+) (pilD) [Klebsiella pneumoniae strain T877-PC]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGAGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGACGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425