Detailed information NTG0655844

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MDE60_RS20625
• Genome accession: NZ_CP092077
• Coordinates: 4235983..4236783 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain P1-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4230983..4241783

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MDE60_RS20590 (MDE60_20565)	gspH	4231139..4231648 (+)	510	WP_021441218.1	type II secretion system minor pseudopilin GspH	-
MDE60_RS20595 (MDE60_20570)	gspI	4231651..4232016 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
MDE60_RS20600 (MDE60_20575)	gspJ	4232013..4232609 (+)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
MDE60_RS20605 (MDE60_20580)	gspK	4232606..4233592 (+)	987	WP_004177385.1	type II secretion system minor pseudopilin GspK	-
MDE60_RS20610 (MDE60_20585)	gspL	4233579..4234772 (+)	1194	WP_080899779.1	type II secretion system protein GspL	-
MDE60_RS20615 (MDE60_20590)	-	4234765..4235250 (+)	486	WP_004151943.1	type II secretion system protein M	-
MDE60_RS20620 (MDE60_20595)	-	4235240..4235986 (+)	747	WP_004145897.1	type II secretion system protein N	-
MDE60_RS20625 (MDE60_20600)	pilD	4235983..4236783 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
MDE60_RS20630 (MDE60_20605)	hrpB	4236831..4239260 (-)	2430	WP_004147115.1	ATP-dependent helicase HrpB	-
MDE60_RS20635 (MDE60_20610)	thpR	4239333..4239869 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
MDE60_RS20640 (MDE60_20615)	sfsA	4239869..4240585 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
MDE60_RS20645 (MDE60_20620)	dksA	4240748..4241203 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=655844 MDE60_RS20625 WP_004145898.1 4235983..4236783(+) (pilD) [Klebsiella pneumoniae strain P1-1]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=655844 MDE60_RS20625 WP_004145898.1 4235983..4236783(+) (pilD) [Klebsiella pneumoniae strain P1-1]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425