Detailed information NTG0654849

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: L6V94_RS10400
• Genome accession: NZ_CP091978
• Coordinates: 2153277..2154077 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae isolate JNQH373
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2148277..2159077

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6V94_RS10380 (L6V94_10380)	dksA	2148857..2149312 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
L6V94_RS10385 (L6V94_10385)	sfsA	2149475..2150191 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
L6V94_RS10390 (L6V94_10390)	thpR	2150191..2150727 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
L6V94_RS10395 (L6V94_10395)	hrpB	2150800..2153229 (+)	2430	WP_012737409.1	ATP-dependent helicase HrpB	-
L6V94_RS10400 (L6V94_10400)	pilD	2153277..2154077 (-)	801	WP_004145898.1	prepilin peptidase	Machinery gene
L6V94_RS10405 (L6V94_10405)	-	2154074..2154820 (-)	747	WP_012737410.1	type II secretion system protein N	-
L6V94_RS10410 (L6V94_10410)	-	2154810..2155295 (-)	486	WP_012737411.1	type II secretion system protein M	-
L6V94_RS10415 (L6V94_10415)	gspL	2155288..2156481 (-)	1194	WP_012737412.1	type II secretion system protein GspL	-
L6V94_RS10420 (L6V94_10420)	gspK	2156468..2157454 (-)	987	WP_077250502.1	type II secretion system minor pseudopilin GspK	-
L6V94_RS10425 (L6V94_10425)	gspJ	2157451..2158047 (-)	597	WP_012737414.1	type II secretion system minor pseudopilin GspJ	-
L6V94_RS10430 (L6V94_10430)	gspI	2158044..2158409 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
L6V94_RS10435 (L6V94_10435)	gspH	2158406..2158921 (-)	516	WP_004183045.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=654849 L6V94_RS10400 WP_004145898.1 2153277..2154077(-) (pilD) [Klebsiella pneumoniae isolate JNQH373]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=654849 L6V94_RS10400 WP_004145898.1 2153277..2154077(-) (pilD) [Klebsiella pneumoniae isolate JNQH373]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425