Detailed information NTG0653723

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: L7H25_RS21200
• Genome accession: NZ_CP091805
• Coordinates: 4336158..4336958 (+)
• Length: 266 a.a.
• NCBI ID: WP_049116597.1
• Uniprot ID: -
• Organism: Klebsiella quasipneumoniae strain 21223
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4331158..4341958

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L7H25_RS21165 (L7H25_21165)	gspH	4331311..4331826 (+)	516	WP_004204416.1	type II secretion system minor pseudopilin GspH	-
L7H25_RS21170 (L7H25_21170)	gspI	4331823..4332188 (+)	366	WP_114465183.1	type II secretion system minor pseudopilin GspI	-
L7H25_RS21175 (L7H25_21175)	gspJ	4332185..4332781 (+)	597	WP_048294187.1	type II secretion system minor pseudopilin GspJ	-
L7H25_RS21180 (L7H25_21180)	gspK	4332778..4333758 (+)	981	WP_077259420.1	type II secretion system minor pseudopilin GspK	-
L7H25_RS21185 (L7H25_21185)	gspL	4333751..4334947 (+)	1197	WP_080841715.1	type II secretion system protein GspL	-
L7H25_RS21190 (L7H25_21190)	-	4334940..4335425 (+)	486	WP_043521988.1	type II secretion system protein M	-
L7H25_RS21195 (L7H25_21195)	-	4335415..4336143 (+)	729	WP_064178512.1	type II secretion system protein N	-
L7H25_RS21200 (L7H25_21200)	pilD	4336158..4336958 (+)	801	WP_049116597.1	prepilin peptidase	Machinery gene
L7H25_RS21205 (L7H25_21205)	hrpB	4337006..4339435 (-)	2430	WP_032455567.1	ATP-dependent helicase HrpB	-
L7H25_RS21210 (L7H25_21210)	thpR	4339508..4340044 (+)	537	WP_004204405.1	RNA 2',3'-cyclic phosphodiesterase	-
L7H25_RS21215 (L7H25_21215)	sfsA	4340044..4340760 (+)	717	WP_004204404.1	DNA/RNA nuclease SfsA	-
L7H25_RS21220 (L7H25_21220)	dksA	4340923..4341378 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29368.95 Da
• Isoelectric Point: 8.8894

>NTDB_id=653723 L7H25_RS21200 WP_049116597.1 4336158..4336958(+) (pilD) [Klebsiella quasipneumoniae strain 21223]
MTTLAALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLAQTADDEPLTLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQARISWSYPLTELTTGLLFILAGALLAPGLALAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQALPQVLLFASASGLMWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=653723 L7H25_RS21200 WP_049116597.1 4336158..4336958(+) (pilD) [Klebsiella quasipneumoniae strain 21223]
ATGACGACATTAGCCGCATTATCGCTGCATTTCCCCCTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCGCGCATGCTGGCCCAGACCGCGGACGACGAGCCGCTAACCCTCAGCA
CGCCGGGCTCATCCTGTCCGCAGTGTCGCCAGCCGATCGCCTGGCGGGACAATATCCCGTTGCTCAGCTTCCTTTGGCTA
GGCCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGACCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGACTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTTCTGCTGATACTGGCGC
GGATTGATGCCCGGACCCAGCTGCTGCCCGATCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTTAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCTATGGCTGGTTATCTGGCACTGTGGTCGGTCTATTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCTCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGCTCTGCCGCAGGTGCTGTTGTTCGCCTCGGCCAGCGGGCTGATGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425