Detailed information NTG0653004

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: L6D56_RS08725
• Genome accession: NZ_CP091650
• Coordinates: 1780813..1781613 (+)
• Length: 266 a.a.
• NCBI ID: WP_021441214.1
• Uniprot ID: A0A4V0HF22
• Organism: Klebsiella pneumoniae strain TAKPN-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1775813..1786613

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6D56_RS08690	gspH	1775969..1776478 (+)	510	WP_021441218.1	type II secretion system minor pseudopilin GspH	-
L6D56_RS08695	gspI	1776481..1776846 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
L6D56_RS08700	gspJ	1776843..1777439 (+)	597	WP_032428541.1	type II secretion system minor pseudopilin GspJ	-
L6D56_RS08705	gspK	1777436..1778422 (+)	987	WP_004183041.1	type II secretion system minor pseudopilin GspK	-
L6D56_RS08710	gspL	1778409..1779602 (+)	1194	WP_021441216.1	type II secretion system protein GspL	-
L6D56_RS08715	-	1779595..1780080 (+)	486	WP_004151943.1	type II secretion system protein M	-
L6D56_RS08720	-	1780070..1780816 (+)	747	WP_021441215.1	type II secretion system protein N	-
L6D56_RS08725	pilD	1780813..1781613 (+)	801	WP_021441214.1	A24 family peptidase	Machinery gene
L6D56_RS08730	hrpB	1781661..1784090 (-)	2430	WP_032428540.1	ATP-dependent helicase HrpB	-
L6D56_RS08735	thpR	1784163..1784699 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
L6D56_RS08740	sfsA	1784699..1785415 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
L6D56_RS08745	dksA	1785578..1786033 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=653004 L6D56_RS08725 WP_021441214.1 1780813..1781613(+) (pilD) [Klebsiella pneumoniae strain TAKPN-1]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
SRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=653004 L6D56_RS08725 WP_021441214.1 1780813..1781613(+) (pilD) [Klebsiella pneumoniae strain TAKPN-1]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
AGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
TCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

3D structure

• AlphaFold DB: A0A4V0HF22

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425