Detailed information NTG0065283

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: BEST7003_RS18360
• Genome accession: NZ_AP012496
• Coordinates: 3673555..3674700 (+)
• Length: 381 a.a.
• NCBI ID: WP_003227549.1
• Uniprot ID: -
• Organism: Bacillus subtilis BEST7003
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3668555..3679700

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BEST7003_RS18335 (BEST7003_3635)	albF	3668571..3669851 (+)	1281	WP_003242974.1	pitrilysin family protein	-
BEST7003_RS18340 (BEST7003_3636)	albG	3669848..3670549 (+)	702	WP_003243391.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
BEST7003_RS18345 (BEST7003_3637)	ywhL	3670555..3671931 (-)	1377	WP_003244415.1	YncE family protein	-
BEST7003_RS18350 (BEST7003_3638)	ywhK	3671970..3673325 (-)	1356	WP_003227552.1	YncE family protein	-
BEST7003_RS22305	-	3673322..3673552 (-)	231	WP_009968328.1	hypothetical protein	-
BEST7003_RS18360 (BEST7003_3639)	rapF	3673555..3674700 (+)	1146	WP_003227549.1	response regulator aspartate phosphatase RapF	Regulator
BEST7003_RS18365 (BEST7003_3640)	phrF	3674684..3674803 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
BEST7003_RS18370 (BEST7003_3641)	ywhH	3674902..3675375 (+)	474	WP_003227547.1	YbaK/EbsC family protein	-
BEST7003_RS18375 (BEST7003_3642)	speB	3675407..3676279 (-)	873	WP_003227545.1	agmatinase	-
BEST7003_RS18380 (BEST7003_3643)	speE	3676340..3677170 (-)	831	WP_003227543.1	spermidine synthase	-
BEST7003_RS18385 (BEST7003_3644)	pbpG	3677372..3679447 (+)	2076	WP_003227540.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45609.09 Da
• Isoelectric Point: 5.1503

>NTDB_id=65283 BEST7003_RS18360 WP_003227549.1 3673555..3674700(+) (rapF) [Bacillus subtilis BEST7003]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKIDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=65283 BEST7003_RS18360 WP_003227549.1 3673555..3674700(+) (rapF) [Bacillus subtilis BEST7003]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATAGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	100	100	1
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572