Detailed information NTG0652171

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GQR96_RS04220
• Genome accession: NZ_CP091527
• Coordinates: 913921..914721 (-)
• Length: 266 a.a.
• NCBI ID: WP_065904237.1
• Uniprot ID: -
• Organism: Klebsiella sp. PCX
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 908921..919721

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GQR96_RS04200 (GQR96_04200)	dksA	909501..909956 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
GQR96_RS04205 (GQR96_04205)	sfsA	910119..910835 (-)	717	WP_004204404.1	DNA/RNA nuclease SfsA	-
GQR96_RS04210 (GQR96_04210)	thpR	910835..911371 (-)	537	WP_004204405.1	RNA 2',3'-cyclic phosphodiesterase	-
GQR96_RS04215 (GQR96_04215)	hrpB	911444..913873 (+)	2430	WP_065904235.1	ATP-dependent helicase HrpB	-
GQR96_RS04220 (GQR96_04220)	pilD	913921..914721 (-)	801	WP_065904237.1	A24 family peptidase	Machinery gene
GQR96_RS04225 (GQR96_04225)	-	914736..915464 (-)	729	WP_065904239.1	type II secretion system protein N	-
GQR96_RS04230 (GQR96_04230)	-	915454..915939 (-)	486	WP_065904241.1	type II secretion system protein M	-
GQR96_RS04235 (GQR96_04235)	gspL	915932..917128 (-)	1197	WP_062914383.1	type II secretion system protein GspL	-
GQR96_RS04240 (GQR96_04240)	gspK	917121..918101 (-)	981	WP_175936600.1	type II secretion system minor pseudopilin GspK	-
GQR96_RS04245 (GQR96_04245)	gspJ	918098..918694 (-)	597	WP_065904245.1	type II secretion system minor pseudopilin GspJ	-
GQR96_RS04250 (GQR96_04250)	gspI	918691..919056 (-)	366	WP_069734362.1	type II secretion system minor pseudopilin GspI	-
GQR96_RS04255 (GQR96_04255)	gspH	919053..919568 (-)	516	WP_004204416.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29390.94 Da
• Isoelectric Point: 8.6098

>NTDB_id=652171 GQR96_RS04220 WP_065904237.1 913921..914721(-) (pilD) [Klebsiella sp. PCX]
MTTLAALSLHFPLIWYGFLLLFGLALGSFYNVVIYRLPRMLAQTADDEPLTLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
DRRARCCQARISWSYPLTELTTGLLFILAGALLAPGLALAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLVASASGLTWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=652171 GQR96_RS04220 WP_065904237.1 913921..914721(-) (pilD) [Klebsiella sp. PCX]
ATGACGACATTAGCCGCATTATCGCTGCATTTCCCTCTAATCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCGCGCATGCTGGCCCAGACCGCGGACGACGAGCCGCTAACCCTCAGCA
CGCCGGGCTCATCCTGTCCGCAGTGTCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGCTTCCTCTGGCTA
GACCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGACCACCGGCCTGCTGTTTAT
CCTCGCGGGGGCGCTGCTCGCACCGGGACTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTTCTGCTGATACTGGCGC
GGATTGATGCCCGGACCCAGCTGCTGCCCGATCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTTAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCGATGGCTGGTTATCTGGCGCTGTGGTCGGTCTATTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCTCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTTTTGGTCGCCTCGGCCAGCGGGCTGACGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-230)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425