Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   L4X60_RS10070 Genome accession   NZ_CP091525
Coordinates   2036456..2038084 (+) Length   542 a.a.
NCBI ID   WP_001186602.1    Uniprot ID   Q9XB94
Organism   Staphylococcus aureus strain N09CSA16     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2030895..2065086 2036456..2038084 within 0
SCCmec 2035085..2056450 2036456..2038084 within 0


Gene organization within MGE regions


Location: 2030895..2065086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L4X60_RS10050 (L4X60_10050) - 2031523..2032569 (+) 1047 WP_001060127.1 hypothetical protein -
  L4X60_RS10055 (L4X60_10055) - 2032762..2033058 (+) 297 WP_000273824.1 cassette chromosome ssDNA-binding protein -
  L4X60_RS10060 (L4X60_10060) - 2033058..2034851 (+) 1794 WP_000195100.1 cassette chromosome replicative helicase -
  L4X60_RS10065 (L4X60_10065) ccrA 2035085..2036434 (+) 1350 WP_000815646.1 cassette chromosome recombinase CcrA Machinery gene
  L4X60_RS10070 (L4X60_10070) ccrB 2036456..2038084 (+) 1629 WP_001186602.1 cassette chromosome recombinase CcrB Machinery gene
  L4X60_RS10075 (L4X60_10075) - 2038602..2038952 (+) 351 WP_000859155.1 SAUGI family uracil-DNA glycosylase inhibitor -
  L4X60_RS10080 (L4X60_10080) - 2039039..2039350 (+) 312 WP_000700853.1 DUF960 family protein -
  L4X60_RS10085 (L4X60_10085) - 2039368..2039874 (+) 507 WP_001092169.1 DUF1643 domain-containing protein -
  L4X60_RS10090 (L4X60_10090) - 2039895..2040212 (+) 318 WP_000659050.1 JAB domain-containing protein -
  L4X60_RS10095 (L4X60_10095) - 2040331..2041416 (+) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  L4X60_RS10100 (L4X60_10100) - 2041413..2043305 (+) 1893 WP_001557544.1 site-specific integrase -
  L4X60_RS10105 (L4X60_10105) - 2043312..2043689 (+) 378 WP_000361059.1 DUF6262 family protein -
  L4X60_RS10110 (L4X60_10110) - 2043840..2044622 (+) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  L4X60_RS10115 (L4X60_10115) erm(A) 2044748..2045479 (-) 732 WP_001072201.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  L4X60_RS14650 - 2045562..2045621 (-) 60 WP_010959190.1 erythromycin resistance leader peptide -
  L4X60_RS10120 (L4X60_10120) - 2046013..2046675 (+) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  L4X60_RS10125 (L4X60_10130) - 2047020..2047658 (-) 639 Protein_1971 sulfite exporter TauE/SafE family protein -
  L4X60_RS10130 (L4X60_10135) cstR 2047658..2047918 (-) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  L4X60_RS10135 (L4X60_10140) - 2048054..2049118 (+) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  L4X60_RS10140 (L4X60_10145) cstB 2049151..2050485 (+) 1335 Protein_1974 persulfide dioxygenase-sulfurtransferase CstB -
  L4X60_RS10145 (L4X60_10150) - 2050599..2051708 (-) 1110 WP_000943131.1 ROK family transcriptional regulator -
  L4X60_RS10150 (L4X60_10155) psm-mec 2052063..2052131 (+) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  L4X60_RS10155 (L4X60_10160) mecI 2052216..2052587 (-) 372 WP_000369214.1 mecA-type methicillin resistance repressor MecI -
  L4X60_RS10160 (L4X60_10165) mecR1 2052587..2054344 (-) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  L4X60_RS10165 (L4X60_10170) mecA 2054441..2056450 (+) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  L4X60_RS10170 (L4X60_10175) - 2056496..2056924 (-) 429 WP_000872606.1 MaoC family dehydratase -
  L4X60_RS10175 (L4X60_10180) - 2057021..2057764 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  L4X60_RS10180 (L4X60_10185) - 2058641..2059030 (-) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  L4X60_RS10185 (L4X60_10190) - 2059066..2059740 (+) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  L4X60_RS10190 (L4X60_10195) aadD1 2059933..2060703 (+) 771 WP_001795128.1 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia -
  L4X60_RS10195 (L4X60_10200) bleO 2060920..2061324 (+) 405 WP_001242578.1 bleomycin binding protein -
  L4X60_RS10200 (L4X60_10205) mobV 2061831..2063093 (+) 1263 WP_000119405.1 MobV family relaxase -
  L4X60_RS10205 (L4X60_10210) - 2063338..2064321 (+) 984 Protein_1987 protein rep -
  L4X60_RS10210 (L4X60_10215) - 2064412..2065086 (+) 675 WP_112401343.1 IS6-like element IS257 family transposase -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62664.84 Da        Isoelectric Point: 9.8600

>NTDB_id=652157 L4X60_RS10070 WP_001186602.1 2036456..2038084(+) (ccrB) [Staphylococcus aureus strain N09CSA16]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRILKDAKEGYIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPNSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKLNNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKPLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=652157 L4X60_RS10070 WP_001186602.1 2036456..2038084(+) (ccrB) [Staphylococcus aureus strain N09CSA16]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAATCGACCTGAGCTCCAACGTATATTGAAGGATGCGAAAGAAGGCTATATCGACTGTGTTATG
GTCTACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTATCAGAGCGTATGGAAGTCAATACTTCATCGGGTAAGCTCATGTTACAAATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAATGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCTAATAGTAAACATGAACTGATGATTAATCAACATGAAGCTAATATTGTGAAATA
TATATTCGAGTCCTATGCCAAAGGCCATGGCTATCGTAAAATAGCCAATGCATTAAATCACAAAGGCTATGTCACTAAAA
AGGGTAAACCTTTTAGTATTAGTTCTATCACATATATATTAGCTAACCCATTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTTAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAAAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACACTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAACTAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGCCTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATAAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTGTTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9XB94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

100

100

1