Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   L5471_RS02525 Genome accession   NZ_CP091523
Coordinates   477343..477840 (+) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain N12HSA28     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 472343..482840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L5471_RS02485 (L5471_02485) - 472387..473373 (+) 987 WP_000161314.1 ROK family glucokinase -
  L5471_RS02490 (L5471_02490) - 473373..473702 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  L5471_RS02495 (L5471_02495) - 473699..474322 (+) 624 WP_001223010.1 MBL fold metallo-hydrolase -
  L5471_RS02500 (L5471_02500) comGA 474374..475348 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  L5471_RS02505 (L5471_02505) comGB 475320..476390 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  L5471_RS02510 (L5471_02510) comGC 476404..476715 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  L5471_RS02515 (L5471_02515) comGD 476693..477139 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  L5471_RS02520 (L5471_02520) comGE 477126..477425 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  L5471_RS02525 (L5471_02525) comGF 477343..477840 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  L5471_RS02530 (L5471_02530) - 477937..478083 (+) 147 WP_001789879.1 hypothetical protein -
  L5471_RS02535 (L5471_02535) - 478073..478597 (+) 525 WP_001015121.1 shikimate kinase -
  L5471_RS02540 (L5471_02540) gcvT 478756..479847 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  L5471_RS02545 (L5471_02545) gcvPA 479867..481213 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  L5471_RS02550 (L5471_02550) gcvPB 481206..482678 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=652096 L5471_RS02525 WP_001789864.1 477343..477840(+) (comGF) [Staphylococcus aureus strain N12HSA28]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=652096 L5471_RS02525 WP_001789864.1 477343..477840(+) (comGF) [Staphylococcus aureus strain N12HSA28]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964