Detailed information NTG0651557

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: L3249_RS23605
• Genome accession: NZ_CP091470
• Coordinates: 4963459..4964256 (+)
• Length: 265 a.a.
• NCBI ID: WP_032694399.1
• Uniprot ID: A0A2J5PD60
• Organism: Klebsiella michiganensis strain KO_408
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4958459..4969256

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L3249_RS23570 (L3249_23600)	gspH	4958600..4959112 (+)	513	WP_014227977.1	type II secretion system minor pseudopilin GspH	-
L3249_RS23575 (L3249_23605)	gspI	4959109..4959474 (+)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
L3249_RS23580 (L3249_23610)	gspJ	4959471..4960067 (+)	597	WP_032694395.1	type II secretion system minor pseudopilin GspJ	-
L3249_RS23585 (L3249_23615)	gspK	4960064..4961044 (+)	981	WP_195378089.1	type II secretion system minor pseudopilin GspK	-
L3249_RS23590 (L3249_23620)	gspL	4961037..4962230 (+)	1194	WP_195378090.1	type II secretion system protein GspL	-
L3249_RS23595 (L3249_23625)	-	4962223..4962708 (+)	486	WP_032748031.1	type II secretion system protein M	-
L3249_RS23600 (L3249_23630)	-	4962698..4963456 (+)	759	WP_038424077.1	type II secretion system protein N	-
L3249_RS23605 (L3249_23635)	pilD	4963459..4964256 (+)	798	WP_032694399.1	A24 family peptidase	Machinery gene
L3249_RS23610 (L3249_23640)	hrpB	4964354..4966783 (-)	2430	WP_195378091.1	ATP-dependent helicase HrpB	-
L3249_RS23615 (L3249_23645)	thpR	4966856..4967392 (+)	537	WP_014837425.1	RNA 2',3'-cyclic phosphodiesterase	-
L3249_RS23620 (L3249_23650)	sfsA	4967392..4968108 (+)	717	WP_004098690.1	DNA/RNA nuclease SfsA	-
L3249_RS23625 (L3249_23655)	dksA	4968273..4968728 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29150.77 Da
• Isoelectric Point: 8.3410

>NTDB_id=651557 L3249_RS23605 WP_032694399.1 4963459..4964256(+) (pilD) [Klebsiella michiganensis strain KO_408]
MENIALLPEFAAQFPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=651557 L3249_RS23605 WP_032694399.1 4963459..4964256(+) (pilD) [Klebsiella michiganensis strain KO_408]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATTCCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTAAATCTCTGCC
TCCCCGCCTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCAGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A2J5PD60

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.808	98.491	0.491
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.273	100	0.449
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434