Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   L3474_RS00145 Genome accession   NZ_CP091450
Coordinates   25171..26532 (+) Length   453 a.a.
NCBI ID   WP_073076903.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 3641/15     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20171..31532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3474_RS00105 (L3474_00100) - 20282..20491 (+) 210 Protein_14 transposase -
  L3474_RS00110 (L3474_00105) - 20526..21323 (-) 798 Protein_15 transposase -
  L3474_RS00115 (L3474_00110) comW 21589..21825 (+) 237 WP_000939544.1 sigma(X)-activator ComW Regulator
  L3474_RS00120 (L3474_00115) - 22056..23342 (+) 1287 WP_000205044.1 adenylosuccinate synthase -
  L3474_RS00125 (L3474_00120) tadA 23543..24010 (+) 468 WP_000291879.1 tRNA adenosine(34) deaminase TadA -
  L3474_RS00135 (L3474_00130) - 24197..24640 (+) 444 WP_000701992.1 dUTP diphosphatase -
  L3474_RS00140 (L3474_00135) - 24642..25157 (+) 516 WP_000691236.1 histidine phosphatase family protein -
  L3474_RS00145 (L3474_00140) radA 25171..26532 (+) 1362 WP_073076903.1 DNA repair protein RadA Machinery gene
  L3474_RS00150 (L3474_00145) - 26605..27102 (+) 498 WP_001809263.1 carbonic anhydrase -
  L3474_RS00155 (L3474_00150) - 27127..27942 (+) 816 WP_000749768.1 PrsW family intramembrane metalloprotease -
  L3474_RS00160 (L3474_00155) - 28087..29055 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  L3474_RS00165 (L3474_00160) - 29189..29470 (-) 282 Protein_25 ISL3 family transposase -
  L3474_RS00170 (L3474_00165) - 29598..30505 (-) 908 Protein_26 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49462.93 Da        Isoelectric Point: 6.1868

>NTDB_id=651228 L3474_RS00145 WP_073076903.1 25171..26532(+) (radA) [Streptococcus pneumoniae strain 3641/15]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINETAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=651228 L3474_RS00145 WP_073076903.1 25171..26532(+) (radA) [Streptococcus pneumoniae strain 3641/15]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTACGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAACTGCTAAACTGGGCTTTACTAAGATTTATGTACCTCAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(63-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

99.558

100

0.996
  radA Streptococcus pneumoniae D39

99.558

100

0.996
  radA Streptococcus pneumoniae R6

99.558

100

0.996
  radA Streptococcus pneumoniae TIGR4

99.558

100

0.996
  radA Streptococcus mitis SK321

99.117

100

0.991
  radA Streptococcus mitis NCTC 12261

99.117

100

0.991
  radA Bacillus subtilis subsp. subtilis str. 168

62.914

100

0.629