Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   LZS83_RS05760 Genome accession   NZ_CP091444
Coordinates   1129547..1133272 (+) Length   1241 a.a.
NCBI ID   WP_242250954.1    Uniprot ID   -
Organism   Bacillus cereus strain MO2     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1124547..1138272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZS83_RS05745 - 1124705..1125298 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  LZS83_RS05750 lepB 1125355..1125918 (+) 564 WP_063224645.1 signal peptidase I -
  LZS83_RS05755 addB 1126035..1129550 (+) 3516 WP_309259837.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  LZS83_RS05760 addA 1129547..1133272 (+) 3726 WP_242250954.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  LZS83_RS05765 - 1133285..1133572 (+) 288 WP_242250956.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  LZS83_RS05770 gerPF 1133627..1133842 (-) 216 WP_001141570.1 spore germination protein GerPF -
  LZS83_RS05775 - 1133885..1134271 (-) 387 WP_063224641.1 spore germination protein GerPE -
  LZS83_RS05780 gerPD 1134287..1134481 (-) 195 WP_001052807.1 spore germination protein GerPD -
  LZS83_RS05785 gerPC 1134488..1135102 (-) 615 WP_242250958.1 spore germination protein GerPC -
  LZS83_RS05790 gerPB 1135170..1135376 (-) 207 WP_063224639.1 spore germination protein GerPB -
  LZS83_RS05795 gerPA 1135391..1135612 (-) 222 WP_001111186.1 spore germination protein GerPA -
  LZS83_RS05800 - 1135709..1135888 (-) 180 WP_063224638.1 aspartyl-phosphate phosphatase Spo0E family protein -
  LZS83_RS05805 - 1136136..1137035 (+) 900 WP_309259838.1 fumarylacetoacetate hydrolase family protein -
  LZS83_RS05810 - 1137069..1137353 (-) 285 WP_063224636.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142722.98 Da        Isoelectric Point: 4.8054

>NTDB_id=651187 LZS83_RS05760 WP_242250954.1 1129547..1133272(+) (addA) [Bacillus cereus strain MO2]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNAIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQIETAEAHI
RKATELAMLPDGPAPRVETLQTDLALLGTLSSAARESWTSVYEAMQSVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVLDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEVYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHIDLKKPITIEVLQEQIAGMVNKELLTFEQAEEITIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=651187 LZS83_RS05760 WP_242250954.1 1129547..1133272(+) (addA) [Bacillus cereus strain MO2]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGAAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAACGGACG
TGATATTTTAGTAGCAGCAGCAGCCGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAACG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCGGCGCAAGAGATGAAAAATAGAATC
GGAGAAGCGTTAGAAAAAGTATTAATTGATGAACCAGGATCTCAGCACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCTATTTCGACGATCCATTCATTTTGTTTACAAGTCATTAGAGGATACTATTACATGCTTGATGTGGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAACGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATGCGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAGGCATACGATGTGGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCGTATTTATTAGAAGATGTGAAATTCCAGATTGAAACGGCGGAAGCGCATATT
CGTAAAGCGACTGAACTTGCAATGCTTCCTGACGGTCCAGCACCTCGCGTTGAAACCCTACAAACAGACTTAGCTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCATGGACAAGTGTGTATGAAGCGATGCAGAGTGTATCGTGGCAAACGTTAA
AACGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTGCAAGAAGAGCTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCAGAGAGTGAAGGAAACTTGTTCATGGTAGGTGACGTGAAGCAGTCGATTTATCGTTTCCGATTAGCAG
AACCAGGACTATTCTTAGGAAAATATAAACGTTTCACGCAAGAAGGATTGGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGCTAGCAGGCACGAACTTCATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTACCCAGAAGGTGAAGATGTAGCGGCTGAGCTATTATGTA
TTCAGCAAACGGAAGAAGAAGTACTAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAACTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTCTTCCGCGTTATCGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTGCTTCGTTCACCGATCGTTGGATTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCACTTGAAGAAGAGCAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAGTCACTTTCTGATTTG
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGATTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCGAGACAATATGAAGCAACATCATTTAGAGGGTTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACAGCGAGAGCTTTAGGTGAACAAGAAGACGTCGTTCGCATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTTCCAGTTGTATTTGTAGCTGGACTTGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCGCAGTTCATTGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTATTATACGTAGCATTAACGCGT
GCAAAAGAGAAGTTAATTTTAATCGGAACAGTGAAAGATGCAACTAAGGAAATGGAGAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCATCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGATGAAGTTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTAGACGGTAACACGTTACTTGCACCAGAGCCAGTTCAAGAAGAGAAGCAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCGCCAATTAAAACACGTCCACGCTTTATGGAGAAGAAAGGGCTAACGTACGCAGAGCGAGG
AACAGCAGTCCATGCCGTTATGCAGCATATTGATTTGAAGAAGCCGATTACGATTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACTTTTGAACAAGCAGAAGAAATTACGATTGAAAAAGTCATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCCTTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGCGACGAAACGATTCTTGTCCAAGGTGTTATCGACTGTATGATTGAAGAGGAAGATG
GCATCACGTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGTGGATTCGATCAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGCTATATGCAAAAGCACTCGAGAAAAGCTTACAACATCCTGTAAAAGAGAAATG
TTTATACTTCTTTGATGGCAATCACGTTATAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1232)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.488

100

0.537