Detailed information NTG0650952

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: L3V45_RS12515
• Genome accession: NZ_CP091421
• Coordinates: 2562272..2562886 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus sp. R45
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2557272..2567886

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L3V45_RS12485 (L3V45_12485)	rpsT	2557572..2557838 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
L3V45_RS12490 (L3V45_12490)	holA	2557855..2558896 (-)	1042	Protein_2415	DNA polymerase III subunit delta	-
L3V45_RS12495 (L3V45_12495)	-	2558936..2559090 (-)	155	Protein_2416	hypothetical protein	-
L3V45_RS12500 (L3V45_12500)	-	2559131..2559265 (+)	135	WP_003152870.1	YqzM family protein	-
L3V45_RS12505 (L3V45_12505)	comEC	2559284..2561635 (-)	2352	WP_234949402.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
L3V45_RS12510 (L3V45_12510)	-	2561636..2562205 (-)	570	WP_003152868.1	ComE operon protein 2	-
L3V45_RS12515 (L3V45_12515)	comEA	2562272..2562886 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
L3V45_RS12520 (L3V45_12520)	comER	2562945..2563766 (+)	822	WP_014305449.1	late competence protein ComER	-
L3V45_RS12525 (L3V45_12525)	-	2563835..2564572 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
L3V45_RS12530 (L3V45_12530)	rsfS	2564569..2564925 (-)	357	WP_003152864.1	ribosome silencing factor	-
L3V45_RS12535 (L3V45_12535)	yqeK	2564922..2565503 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
L3V45_RS12540 (L3V45_12540)	-	2565493..2566062 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
L3V45_RS12545 (L3V45_12545)	yhbY	2566073..2566363 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
L3V45_RS12550 (L3V45_12550)	aroE	2566357..2567199 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=650952 L3V45_RS12515 WP_043867297.1 2562272..2562886(-) (comEA) [Bacillus sp. R45]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=650952 L3V45_RS12515 WP_043867297.1 2562272..2562886(-) (comEA) [Bacillus sp. R45]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363