Detailed information NTG0650360

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: L2Z13_RS09165
• Genome accession: NZ_CP091361
• Coordinates: 1886828..1887886 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain AB177-VUB
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1881828..1892886

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Z13_RS09140 (L2Z13_09140)	aroK	1882098..1882667 (-)	570	WP_000643712.1	shikimate kinase AroK	-
L2Z13_RS09145 (L2Z13_09145)	pilQ	1882679..1884844 (-)	2166	WP_029424115.1	type IV pilus secretin PilQ family protein	Machinery gene
L2Z13_RS09150 (L2Z13_09150)	pilP	1884912..1885439 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
L2Z13_RS09155 (L2Z13_09155)	pilO	1885450..1886190 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
L2Z13_RS09160 (L2Z13_09160)	pilN	1886187..1886828 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
L2Z13_RS09165 (L2Z13_09165)	pilM	1886828..1887886 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
L2Z13_RS09170 (L2Z13_09170)	ponA	1888048..1890603 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
L2Z13_RS09175 (L2Z13_09175)	-	1890621..1891445 (-)	825	WP_086221410.1	putative RNA methyltransferase	-
L2Z13_RS09180 (L2Z13_09180)	-	1891448..1891780 (-)	333	WP_000169602.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=650360 L2Z13_RS09165 WP_002014066.1 1886828..1887886(-) (pilM) [Acinetobacter baumannii strain AB177-VUB]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=650360 L2Z13_RS09165 WP_002014066.1 1886828..1887886(-) (pilM) [Acinetobacter baumannii strain AB177-VUB]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAACGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCCGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCATTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGATCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423