Detailed information NTG0649239

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: L3Y35_RS15320
• Genome accession: NZ_CP091330
• Coordinates: 3170831..3171631 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRK3022
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3165831..3176631

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L3Y35_RS15285	gspH	3165987..3166502 (+)	516	WP_004194939.1	type II secretion system minor pseudopilin GspH	-
L3Y35_RS15290	gspI	3166499..3166864 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
L3Y35_RS15295	gspJ	3166861..3167457 (+)	597	WP_004178612.1	type II secretion system minor pseudopilin GspJ	-
L3Y35_RS15300	gspK	3167454..3168440 (+)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
L3Y35_RS15305	gspL	3168427..3169620 (+)	1194	WP_004194919.1	type II secretion system protein GspL	-
L3Y35_RS15310	-	3169613..3170098 (+)	486	WP_004145896.1	type II secretion system protein M	-
L3Y35_RS15315	-	3170088..3170834 (+)	747	WP_004178605.1	type II secretion system protein N	-
L3Y35_RS15320	pilD	3170831..3171631 (+)	801	WP_004145898.1	A24 family peptidase	Machinery gene
L3Y35_RS15325	hrpB	3171679..3174108 (-)	2430	WP_249232951.1	ATP-dependent helicase HrpB	-
L3Y35_RS15330	thpR	3174181..3174717 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
L3Y35_RS15335	sfsA	3174717..3175433 (+)	717	WP_020324420.1	DNA/RNA nuclease SfsA	-
L3Y35_RS15340	dksA	3175596..3176051 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=649239 L3Y35_RS15320 WP_004145898.1 3170831..3171631(+) (pilD) [Klebsiella pneumoniae strain CRK3022]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=649239 L3Y35_RS15320 WP_004145898.1 3170831..3171631(+) (pilD) [Klebsiella pneumoniae strain CRK3022]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCATGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425