Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   L2Z53_RS06985 Genome accession   NZ_CP091281
Coordinates   1355374..1356345 (-) Length   323 a.a.
NCBI ID   WP_236365614.1    Uniprot ID   -
Organism   Macrococcoides canis strain B8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1350374..1361345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Z53_RS06950 (L2Z53_06950) gcvT 1351071..1352156 (-) 1086 WP_086043664.1 glycine cleavage system aminomethyltransferase GcvT -
  L2Z53_RS06955 (L2Z53_06955) - 1352294..1352800 (-) 507 WP_236365608.1 shikimate kinase -
  L2Z53_RS06960 (L2Z53_06960) - 1352961..1353413 (-) 453 WP_236365609.1 competence type IV pilus minor pilin ComGF -
  L2Z53_RS06965 (L2Z53_06965) - 1353358..1353600 (-) 243 WP_236365610.1 hypothetical protein -
  L2Z53_RS06970 (L2Z53_06970) comGD 1353587..1354033 (-) 447 WP_236365611.1 competence type IV pilus minor pilin ComGD -
  L2Z53_RS06975 (L2Z53_06975) comGC 1354005..1354337 (-) 333 WP_236365612.1 competence type IV pilus major pilin ComGC -
  L2Z53_RS06980 (L2Z53_06980) comGB 1354350..1355360 (-) 1011 WP_236365613.1 competence type IV pilus assembly protein ComGB -
  L2Z53_RS06985 (L2Z53_06985) comGA 1355374..1356345 (-) 972 WP_236365614.1 competence type IV pilus ATPase ComGA Machinery gene
  L2Z53_RS06990 (L2Z53_06990) - 1356557..1356805 (+) 249 WP_086042733.1 DUF2626 domain-containing protein -
  L2Z53_RS06995 (L2Z53_06995) - 1356833..1357453 (-) 621 WP_236365615.1 MBL fold metallo-hydrolase -
  L2Z53_RS07000 (L2Z53_07000) - 1357543..1357782 (+) 240 WP_174804788.1 DUF2759 domain-containing protein -
  L2Z53_RS07005 (L2Z53_07005) - 1357809..1358120 (-) 312 WP_086042736.1 MTH1187 family thiamine-binding protein -
  L2Z53_RS07010 (L2Z53_07010) - 1358120..1359088 (-) 969 WP_236365616.1 ROK family glucokinase -
  L2Z53_RS07015 (L2Z53_07015) - 1359085..1359276 (-) 192 WP_086042738.1 YqgQ family protein -
  L2Z53_RS07020 (L2Z53_07020) - 1359281..1360708 (-) 1428 WP_236365617.1 rhomboid family intramembrane serine protease -
  L2Z53_RS07025 (L2Z53_07025) - 1360705..1361259 (-) 555 WP_236365618.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 37271.88 Da        Isoelectric Point: 6.7240

>NTDB_id=648822 L2Z53_RS06985 WP_236365614.1 1355374..1356345(-) (comGA) [Macrococcoides canis strain B8]
MEKLFNEIIEEAIIQGASDIHFIPNDKSISIKFRIHGDIEDYREIDDMLFRKLLSYIKFTAHLDVSEKNKAQSGIIHFNL
KDLRYNIRASTLPRALGDEACVLRIIRQNFIDEYQTDDKILFNQIKKSSGIIIISGPTGSGKSTLMYQLVHYARNTLKRQ
IISIEDPVEQHIEGIIQVNVNEKAEITYHTAIKAILRCDPDIIMLGEIRDSVVAQQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINTVDLYQSINLIINQRLVRKKDGKGRLLTYEFLTKKDIEKYLQGEHINYRTLTDQFKELYETNQISHYEFEK
FDL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=648822 L2Z53_RS06985 WP_236365614.1 1355374..1356345(-) (comGA) [Macrococcoides canis strain B8]
ATGGAAAAATTATTCAATGAAATAATAGAGGAAGCAATTATACAAGGTGCATCAGATATTCACTTCATACCAAACGATAA
AAGCATATCTATCAAATTTAGGATACATGGTGATATTGAAGACTATAGAGAAATCGACGACATGTTATTCAGGAAATTAC
TTTCTTATATTAAATTTACGGCACATCTTGACGTATCAGAAAAGAATAAGGCACAGAGTGGAATCATCCATTTTAATCTG
AAAGACTTAAGATATAATATTCGCGCATCTACTTTACCTCGAGCATTAGGCGATGAAGCATGTGTACTGAGAATCATCAG
ACAAAATTTTATTGATGAATATCAGACGGATGACAAGATATTGTTCAATCAAATAAAAAAATCAAGCGGTATTATCATCA
TAAGTGGACCAACTGGAAGCGGGAAAAGCACATTGATGTACCAGCTTGTACATTATGCGAGGAACACATTGAAGAGACAA
ATAATATCAATAGAAGATCCTGTTGAACAGCATATTGAAGGAATTATACAAGTTAATGTTAACGAAAAAGCAGAGATAAC
ATATCATACCGCGATAAAGGCAATCCTTAGATGTGATCCGGATATCATTATGCTAGGTGAAATCAGAGATTCAGTCGTAG
CACAGCAAGTGATCAATGCAGGACTAAGCGGTCATCTCGTTTTAACGACATTACATGCGAATGATTGCATCGGTGCATTA
TTTCGGTTAAAAGAAATGGGTATTAATACTGTTGATCTATATCAAAGTATCAATCTGATTATTAATCAAAGGCTTGTAAG
AAAGAAAGATGGGAAGGGGCGTCTATTGACCTATGAATTTTTGACAAAAAAGGATATTGAAAAATATTTACAAGGTGAAC
ATATTAACTATAGAACGTTGACCGATCAATTTAAGGAGCTCTATGAAACAAATCAGATTTCACACTATGAATTCGAAAAA
TTTGATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

48.78

100

0.495
  comGA Staphylococcus aureus N315

48.78

100

0.495
  comGA Bacillus subtilis subsp. subtilis str. 168

38.037

100

0.384
  pilB Glaesserella parasuis strain SC1401

34.012

100

0.362
  pilB Vibrio parahaemolyticus RIMD 2210633

34.513

100

0.362