Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L1999_RS22515 Genome accession   NZ_CP091110
Coordinates   4709021..4710070 (-) Length   349 a.a.
NCBI ID   WP_235740594.1    Uniprot ID   -
Organism   Neobacillus drentensis strain JC05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4704021..4715070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1999_RS22490 (L1999_22490) - 4704584..4705423 (-) 840 WP_235740590.1 PilN domain-containing protein -
  L1999_RS22495 (L1999_22495) pilM 4705420..4706418 (-) 999 WP_235740591.1 pilus assembly protein PilM -
  L1999_RS22500 (L1999_22500) comC 4706446..4707189 (-) 744 WP_235740592.1 A24 family peptidase Machinery gene
  L1999_RS29710 - 4707189..4707668 (-) 480 WP_275677205.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  L1999_RS22510 (L1999_22510) - 4707823..4709028 (-) 1206 WP_235740593.1 type II secretion system F family protein -
  L1999_RS22515 (L1999_22515) pilT 4709021..4710070 (-) 1050 WP_235740594.1 type IV pilus twitching motility protein PilT Machinery gene
  L1999_RS22520 (L1999_22520) - 4710081..4711772 (-) 1692 WP_235740595.1 ATPase, T2SS/T4P/T4SS family -
  L1999_RS22525 (L1999_22525) - 4711756..4713072 (-) 1317 WP_235740596.1 G5 domain-containing protein -
  L1999_RS22530 (L1999_22530) - 4713095..4714222 (-) 1128 WP_235740597.1 hypothetical protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 39030.07 Da        Isoelectric Point: 8.3956

>NTDB_id=647801 L1999_RS22515 WP_235740594.1 4709021..4710070(-) (pilT) [Neobacillus drentensis strain JC05]
MNERIDNILRAAMEFRASDIHLTVGVQPIFRINGDIKRYGKEILLPADTEAIAKQIIPENLYEQFKERGELDFSYGIPGV
SRFRVNAYHQRKSVSLALRVVASKTPTLEDLDLPEIIPKLVEKPQGLILVTGPTGSGKSTTLASMVDYMNRTMRKHIITL
EDPIEYLHKHGNSIIDQREVGFDTKNYASGLRAALRQDPDVILVGEMRDLETIGIAITAAETGHLVFGTLHTSSAPATIN
RIIDVFPPAQQPQIRVQLASVLVGVISQRLFPTVDKQGRKAATEILVNNPAIANLIRSEKIHQIQSTMQTSRAQGMHTLE
MSIRDMLDRNLIQKESATKYLQEKMMMDA

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=647801 L1999_RS22515 WP_235740594.1 4709021..4710070(-) (pilT) [Neobacillus drentensis strain JC05]
ATGAATGAAAGAATTGATAACATTCTACGGGCCGCAATGGAATTCAGAGCATCAGATATCCATTTAACCGTTGGAGTACA
ACCCATTTTTCGTATTAATGGAGATATTAAACGCTATGGGAAAGAGATATTGTTACCAGCAGACACGGAGGCAATTGCCA
AACAAATTATCCCCGAAAACTTATATGAACAATTTAAAGAACGAGGGGAACTTGATTTCTCATATGGGATCCCTGGAGTA
TCCCGGTTTCGTGTCAATGCCTATCATCAACGTAAAAGTGTTTCTCTTGCTTTAAGGGTAGTTGCGTCAAAAACTCCGAC
ACTTGAAGATTTGGATTTACCGGAAATCATTCCAAAGCTTGTTGAAAAGCCGCAAGGATTAATCCTTGTGACAGGGCCTA
CAGGAAGTGGTAAGTCGACAACACTTGCCTCGATGGTAGATTATATGAATCGCACAATGAGGAAGCATATCATTACCCTA
GAAGATCCAATTGAGTATCTTCATAAACATGGAAATTCCATTATTGATCAGCGCGAGGTGGGATTTGATACTAAGAACTA
TGCGAGTGGTCTAAGAGCAGCCCTTCGTCAAGATCCCGATGTTATTCTTGTAGGGGAGATGCGTGACTTAGAGACGATTG
GTATTGCAATCACAGCAGCCGAAACGGGTCACTTAGTTTTTGGTACCCTTCATACCTCAAGTGCACCGGCAACCATTAAC
CGTATTATTGATGTATTTCCACCAGCACAGCAGCCACAAATACGTGTCCAGTTAGCTTCAGTATTGGTGGGGGTTATTTC
ACAGCGTCTATTTCCCACAGTGGATAAACAAGGACGAAAAGCTGCAACGGAGATATTAGTGAATAATCCTGCGATCGCTA
ACTTAATTAGAAGTGAAAAGATTCACCAAATTCAAAGTACGATGCAAACTTCACGCGCCCAAGGAATGCATACGCTGGAG
ATGAGTATTAGAGACATGCTTGATCGTAATTTGATTCAGAAAGAATCAGCAACTAAATATCTCCAAGAAAAGATGATGAT
GGATGCCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.134

93.983

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.093

98.567

0.464
  pilT Vibrio cholerae strain A1552

47.093

98.567

0.464
  pilT Neisseria meningitidis 8013

45.64

98.567

0.45
  pilT Pseudomonas stutzeri DSM 10701

48.91

91.977

0.45
  pilT Neisseria gonorrhoeae MS11

45.349

98.567

0.447
  pilT Acinetobacter baumannii D1279779

46.547

95.415

0.444
  pilT Acinetobacter nosocomialis M2

46.547

95.415

0.444
  pilT Acinetobacter baumannii strain A118

46.547

95.415

0.444
  pilT Pseudomonas aeruginosa PAK

48.287

91.977

0.444
  pilT Acinetobacter baylyi ADP1

45.946

95.415

0.438
  pilT Legionella pneumophila strain ERS1305867

45.345

95.415

0.433
  pilT Legionella pneumophila strain Lp02

45.345

95.415

0.433
  pilU Acinetobacter baylyi ADP1

40.294

97.421

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.64

95.415

0.378
  pilU Vibrio cholerae strain A1552

38.576

96.562

0.372