Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   H7X62_RS22290 Genome accession   NZ_AP021935
Coordinates   4739608..4741806 (+) Length   732 a.a.
NCBI ID   WP_001550368.1    Uniprot ID   -
Organism   Escherichia coli strain WP2-W18-ESBL-10     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4734608..4746806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7X62_RS22280 (WP2W18E10_43190) - 4734805..4739033 (-) 4229 Protein_4401 RHS element core protein -
  H7X62_RS22285 (WP2W18E10_43200) rpmE 4739193..4739405 (-) 213 WP_000710769.1 50S ribosomal protein L31 -
  H7X62_RS22290 (WP2W18E10_43210) priA 4739608..4741806 (+) 2199 WP_001550368.1 primosomal protein N' Machinery gene
  H7X62_RS22295 (WP2W18E10_43220) cytR 4741962..4742987 (+) 1026 WP_000644908.1 DNA-binding transcriptional regulator CytR Regulator
  H7X62_RS22300 (WP2W18E10_43230) ftsN 4743079..4744038 (+) 960 WP_000068834.1 cell division protein FtsN -
  H7X62_RS22305 (WP2W18E10_43240) hslV 4744131..4744661 (+) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  H7X62_RS22310 (WP2W18E10_43250) hslU 4744671..4746002 (+) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81694.10 Da        Isoelectric Point: 9.2620

>NTDB_id=64674 H7X62_RS22290 WP_001550368.1 4739608..4741806(+) (priA) [Escherichia coli strain WP2-W18-ESBL-10]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAMLEF
NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTHRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=64674 H7X62_RS22290 WP_001550368.1 4739608..4741806(+) (priA) [Escherichia coli strain WP2-W18-ESBL-10]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCCCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTCAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCAATGTCAGCG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGATTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCACCCAAGCAACAACAGGCGCTGGCAGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTTGCCATGCTCGAATTT
AATGATGCCGCGTTGCAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGTGACTGGCG
AAAGAATTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACTGAACAGGCCACCGCCGTTGGCGCAATTCATAGTGCGG
CGGATACCTTCTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACGCCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGCGAGGCGGCGATTGTCATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTTATTGAT
GAAGAGCACGACAGTTCCTACAAACAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGCGCGCATAG
CGAGCAAATCCCAATTATTCTTGGCTCCGCAACGCCCGCACTGGAAACGTTATGCAACGTGCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACGCATCGGGCGGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCTGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCAAACAATCAGGTTATCCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGCCTTGCTGTGCCACGACTGCGGCTGGATTGCCGAATGTCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGCCACCATTGCGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACACTCGCGCCGTTGTTCCCTGGTGTCCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGCGCACTGGAACAACAACTGGCGGAAGTACATCGCGGTGGCGCGA
GGATTTTGATCGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCGTTACTGGACGTAGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCCCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTCTTGCAAACATTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAACGACGCATGATGCAACTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCGCTGTTCCTGCAACAACTGCGTAATCTGATCCTCTCCAGCCCGCTGGCAGA
CGACAAACTGTGGGTTCTCGGTCCCGTTCCGGCACTGGCACCTAAACGTGGCGGCCGCTGGCGCTGGCAAATTTTGTTGC
AGCACCCTTCCCGCGTGCGATTACAGCACATCATCAATGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTCGATCCCATCGAGGGCTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

DEAD/DEAH box helicase (DEAD)

(203-362)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.64

100

0.449

Multiple sequence alignment