Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   L1A18_RS10395 Genome accession   NZ_CP090887
Coordinates   2029818..2031116 (-) Length   432 a.a.
NCBI ID   WP_000867612.1    Uniprot ID   A0A0E9GXK4
Organism   Streptococcus pneumoniae strain NP7536     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2024818..2036116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1A18_RS10375 (L1A18_10375) rplI 2025973..2026425 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  L1A18_RS10380 (L1A18_10380) - 2026422..2028395 (-) 1974 WP_000715121.1 DHH family phosphoesterase -
  L1A18_RS10385 (L1A18_10385) raiA 2028531..2029079 (-) 549 WP_000599107.1 ribosome-associated translation inhibitor RaiA -
  L1A18_RS10390 (L1A18_10390) comFC/cflB 2029159..2029821 (-) 663 WP_000649964.1 ComF family protein Machinery gene
  L1A18_RS10395 (L1A18_10395) comFA/cflA 2029818..2031116 (-) 1299 WP_000867612.1 DEAD/DEAH box helicase Machinery gene
  L1A18_RS10400 (L1A18_10400) - 2031172..2031807 (+) 636 WP_000395739.1 YigZ family protein -
  L1A18_RS10405 (L1A18_10405) cysK 2032092..2033012 (+) 921 WP_000029867.1 cysteine synthase A -
  L1A18_RS10410 (L1A18_10410) - 2033251..2033616 (-) 366 Protein_2028 IS66 family transposase -
  L1A18_RS10415 (L1A18_10415) - 2033598..2033885 (+) 288 Protein_2029 transposase -
  L1A18_RS10420 (L1A18_10420) tnpB 2034040..2034363 (-) 324 WP_000973415.1 IS66 family insertion sequence element accessory protein TnpB -
  L1A18_RS10425 (L1A18_10425) tsf 2034609..2035649 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49563.48 Da        Isoelectric Point: 8.4382

>NTDB_id=646019 L1A18_RS10395 WP_000867612.1 2029818..2031116(-) (comFA/cflA) [Streptococcus pneumoniae strain NP7536]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=646019 L1A18_RS10395 WP_000867612.1 2029818..2031116(-) (comFA/cflA) [Streptococcus pneumoniae strain NP7536]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATTAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E9GXK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae D39

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae R6

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae TIGR4

99.074

100

0.991
  comFA/cflA Streptococcus mitis NCTC 12261

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387