Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SPB_RS05455 Genome accession   NZ_AEUT02000001
Coordinates   1087072..1088376 (-) Length   434 a.a.
NCBI ID   WP_003104831.1    Uniprot ID   F1Z2Q1
Organism   Streptococcus parauberis NCFD 2020     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1082072..1093376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPB_RS05445 (SPB_1128) raiA 1085788..1086336 (-) 549 WP_003105371.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SPB_RS05450 (SPB_1129) - 1086411..1087082 (-) 672 WP_254655069.1 ComF family protein -
  SPB_RS05455 (SPB_1130) comFA/cflA 1087072..1088376 (-) 1305 WP_003104831.1 DEAD/DEAH box helicase Machinery gene
  SPB_RS05460 (SPB_1131) - 1088433..1089065 (+) 633 WP_003105463.1 YigZ family protein -
  SPB_RS05465 (SPB_1132) cysK 1089188..1090117 (+) 930 WP_003103108.1 cysteine synthase A -
  SPB_RS05470 (SPB_1133) - 1090193..1090546 (-) 354 WP_003103672.1 S1 RNA-binding domain-containing protein -
  SPB_RS05475 (SPB_1134) - 1090546..1091946 (-) 1401 WP_003104279.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  SPB_RS05480 (SPB_1135) - 1091979..1092620 (-) 642 WP_003105505.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 434 a.a.        Molecular weight: 49737.71 Da        Isoelectric Point: 9.6231

>NTDB_id=64544 SPB_RS05455 WP_003104831.1 1087072..1088376(-) (comFA/cflA) [Streptococcus parauberis NCFD 2020]
MGKNYYEQGRLFTEQQMKNVITAKTFSMKESMSMESGKQCCNRCFQEVAKTNLLPNGKTYCRFCIAFGRVESESQLYYFA
PQAFPKGDYLRWNGQLTPYQEKISEFLLGNYLNKKNSLVHAVTGAGKTEMIYKLIESVINQGGWVCFTSPRVDVCIDIKN
RLVRDFSCPITIMYGGSEEYRPAPLIVSTTHQLLKFYRAFDLLIIDEVDAFPFFGNVVLNKATQSCLKEEGKVVCLTATS
TNELEQKVSDGHYEKIRLSRRFHNHPLVVPKFQLIYPLSKQFEKNKFPNLLSNLISQQRQTAFPLLIFYPLITEGEKFTK
ILQKAFPKEKIAFVSSKSDDRKEKIDAFKKRQIDILVTTTILERGVTFPEVDVYVILANHKLFTSSSLIQIAGRVGRSVS
RPSGQLLFIHEGVSLQMKKARKQIIEMNKEAYGE

Nucleotide


Download         Length: 1305 bp        

>NTDB_id=64544 SPB_RS05455 WP_003104831.1 1087072..1088376(-) (comFA/cflA) [Streptococcus parauberis NCFD 2020]
ATGGGAAAAAATTATTATGAACAAGGTCGACTTTTTACTGAACAACAAATGAAAAATGTGATAACTGCGAAAACTTTTTC
CATGAAAGAAAGTATGAGTATGGAGTCTGGTAAGCAATGTTGCAATAGATGTTTTCAAGAAGTTGCTAAAACTAATCTTT
TACCTAATGGGAAGACATATTGTAGATTTTGTATTGCCTTTGGTCGGGTGGAATCTGAATCACAACTCTATTATTTTGCA
CCTCAAGCATTTCCGAAAGGTGATTATTTACGCTGGAATGGTCAACTAACCCCATATCAAGAAAAAATTTCAGAATTTTT
GTTAGGCAATTATCTTAATAAAAAAAACAGCTTAGTCCATGCTGTGACAGGTGCTGGAAAAACAGAAATGATATATAAAT
TAATTGAATCAGTAATCAATCAAGGTGGCTGGGTCTGTTTTACTAGTCCCCGAGTAGATGTCTGTATTGATATCAAAAAT
CGTTTAGTTCGAGACTTCTCTTGCCCAATAACAATTATGTATGGAGGGTCCGAGGAATATCGTCCAGCCCCCTTGATAGT
TTCAACAACACATCAACTCTTAAAATTTTATCGAGCCTTTGATTTATTAATAATAGATGAAGTTGATGCTTTTCCTTTTT
TTGGGAATGTGGTTCTAAATAAAGCAACACAATCATGTTTAAAAGAGGAAGGTAAAGTTGTTTGTCTGACTGCAACTTCG
ACAAATGAATTAGAACAAAAAGTCAGCGATGGACATTATGAAAAAATAAGATTGTCAAGACGTTTCCATAATCATCCATT
GGTCGTTCCTAAGTTTCAATTAATTTATCCATTATCAAAACAGTTTGAAAAAAATAAATTTCCTAACTTACTAAGTAATT
TGATTAGTCAACAAAGACAGACTGCATTCCCTTTACTAATTTTCTACCCTCTAATTACAGAAGGGGAAAAATTTACGAAA
ATATTGCAAAAAGCTTTTCCTAAAGAAAAAATTGCCTTTGTATCAAGTAAATCTGATGATCGAAAAGAGAAAATTGATGC
GTTTAAAAAAAGACAAATTGATATTCTTGTAACTACGACTATTTTGGAGAGGGGAGTAACATTTCCAGAAGTAGATGTTT
ACGTTATTTTGGCAAATCATAAGCTATTTACGAGTAGTAGCCTGATTCAGATAGCAGGTCGCGTAGGGAGATCCGTATCT
CGACCAAGTGGGCAGTTACTATTTATTCATGAAGGGGTAAGTCTACAAATGAAGAAAGCTCGTAAACAAATTATAGAAAT
GAATAAGGAAGCATATGGTGAATAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(95-207)

Helicase conserved C-terminal domain (Helicase_C)

(289-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F1Z2Q1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae D39

53.052

98.157

0.521
  comFA/cflA Streptococcus pneumoniae R6

53.052

98.157

0.521
  comFA/cflA Streptococcus pneumoniae TIGR4

53.052

98.157

0.521
  comFA/cflA Streptococcus pneumoniae Rx1

53.052

98.157

0.521
  comFA/cflA Streptococcus mitis SK321

52.347

98.157

0.514
  comFA/cflA Streptococcus mitis NCTC 12261

51.643

98.157

0.507
  comFA Lactococcus lactis subsp. cremoris KW2

48.744

91.705

0.447
  comFA Latilactobacillus sakei subsp. sakei 23K

35.202

100

0.362

Multiple sequence alignment