Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   L0999_RS00420 Genome accession   NZ_CP090870
Coordinates   77419..78105 (-) Length   228 a.a.
NCBI ID   WP_001802022.1    Uniprot ID   A0A7U7EYP5
Organism   Staphylococcus aureus strain SAUR_BFS62     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 72419..83105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0999_RS00400 (L0999_00400) rpsT 73333..73584 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  L0999_RS00405 (L0999_00405) holA 73629..74603 (-) 975 WP_001282563.1 DNA polymerase III subunit delta -
  L0999_RS00410 (L0999_00410) comEC 74660..76861 (-) 2202 WP_180993270.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  L0999_RS00415 (L0999_00415) - 76866..77327 (-) 462 WP_000439693.1 ComE operon protein 2 -
  L0999_RS00420 (L0999_00420) comEA 77419..78105 (-) 687 WP_001802022.1 ComEA family DNA-binding protein Machinery gene
  L0999_RS00425 (L0999_00425) - 78145..78861 (-) 717 WP_000084829.1 class I SAM-dependent DNA methyltransferase -
  L0999_RS00430 (L0999_00430) rsfS 78864..79217 (-) 354 WP_001088020.1 ribosome silencing factor -
  L0999_RS00435 (L0999_00435) yqeK 79218..79802 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  L0999_RS00440 (L0999_00440) nadD 79792..80361 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  L0999_RS00445 (L0999_00445) yhbY 80364..80654 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  L0999_RS00450 (L0999_00450) aroE 80658..81464 (-) 807 WP_000666757.1 shikimate dehydrogenase -
  L0999_RS00455 (L0999_00455) yqeH 81478..82578 (-) 1101 WP_001280137.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25900.65 Da        Isoelectric Point: 9.6751

>NTDB_id=645294 L0999_RS00420 WP_001802022.1 77419..78105(-) (comEA) [Staphylococcus aureus strain SAUR_BFS62]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENNSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=645294 L0999_RS00420 WP_001802022.1 77419..78105(-) (comEA) [Staphylococcus aureus strain SAUR_BFS62]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAGTGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAATAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCGAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EYP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus N315

100

100

1
  comEA Staphylococcus aureus MW2

97.368

100

0.974