Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SCV20265_RS25555 Genome accession   NC_023149
Coordinates   5473450..5474322 (+) Length   290 a.a.
NCBI ID   WP_003116251.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa SCV20265     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5468450..5479322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCV20265_RS25535 - 5468993..5469691 (-) 699 WP_023122772.1 hypothetical protein -
  SCV20265_RS32390 - 5469755..5470294 (-) 540 WP_003116248.1 pilin -
  SCV20265_RS25545 (SCV20265_5154) pilB 5470523..5472226 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SCV20265_RS25550 (SCV20265_5155) pilC 5472230..5473447 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  SCV20265_RS25555 (SCV20265_5156) pilD 5473450..5474322 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  SCV20265_RS25560 (SCV20265_5157) coaE 5474319..5474930 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  SCV20265_RS25565 (SCV20265_5158) yacG 5474927..5475127 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  SCV20265_RS25570 (SCV20265_5159) - 5475164..5475373 (-) 210 WP_003094660.1 hypothetical protein -
  SCV20265_RS25575 (SCV20265_5160) - 5475479..5476168 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  SCV20265_RS25580 (SCV20265_5161) - 5476165..5476635 (-) 471 WP_003103866.1 hypothetical protein -
  SCV20265_RS25585 (SCV20265_5162) - 5476632..5477057 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  SCV20265_RS25590 (SCV20265_5163) - 5477190..5477819 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  SCV20265_RS25595 (SCV20265_5164) - 5477816..5478265 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  SCV20265_RS25600 (SCV20265_5165) - 5478291..5478461 (+) 171 WP_023122773.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31871.89 Da        Isoelectric Point: 7.3454

>NTDB_id=64342 SCV20265_RS25555 WP_003116251.1 5473450..5474322(+) (pilD) [Pseudomonas aeruginosa SCV20265]
MELLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=64342 SCV20265_RS25555 WP_003116251.1 5473450..5474322(+) (pilD) [Pseudomonas aeruginosa SCV20265]
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCTATCCTCCTTGGCCTGCTGGTTGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTACCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCTTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.017

99.655

0.548
  pilD Vibrio campbellii strain DS40M4

53.497

98.621

0.528
  pilD Acinetobacter nosocomialis M2

50.352

97.931

0.493
  pilD Acinetobacter baumannii D1279779

50.352

97.931

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472

Multiple sequence alignment