Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   N5938_RS10020 Genome accession   NZ_CP107257
Coordinates   2153204..2153629 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 2019CK-00034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2148204..2158629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5938_RS10000 (N5938_10000) ileS 2148361..2151192 (+) 2832 WP_003094730.1 isoleucine--tRNA ligase -
  N5938_RS10005 (N5938_10005) lspA 2151185..2151694 (+) 510 WP_003110420.1 signal peptidase II -
  N5938_RS10010 (N5938_10010) fkpB 2151687..2152127 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  N5938_RS10015 (N5938_10015) ispH 2152213..2153157 (+) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  N5938_RS10020 (N5938_10020) comF 2153204..2153629 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  N5938_RS10025 (N5938_10025) pilY2 2153626..2153974 (-) 349 Protein_1971 type 4a fimbrial biogenesis protein PilY2 -
  N5938_RS10030 (N5938_10030) pilY1 2153976..2157467 (-) 3492 Protein_1972 type 4a pilus biogenesis protein PilY1 -
  N5938_RS10035 (N5938_10035) pilX 2157479..2158066 (-) 588 WP_034037187.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=642730 N5938_RS10020 WP_003094721.1 2153204..2153629(-) (comF) [Pseudomonas aeruginosa strain 2019CK-00034]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=642730 N5938_RS10020 WP_003094721.1 2153204..2153629(-) (comF) [Pseudomonas aeruginosa strain 2019CK-00034]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383