Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   U722_RS16465 Genome accession   NC_023073
Coordinates   3429527..3430912 (-) Length   461 a.a.
NCBI ID   WP_024085865.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens LFB112     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3424527..3435912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U722_RS16430 (U722_17465) flgL 3424702..3425619 (-) 918 WP_003151396.1 flagellar hook-associated protein FlgL -
  U722_RS16435 (U722_17470) flgK 3425631..3427148 (-) 1518 WP_024085862.1 flagellar hook-associated protein FlgK -
  U722_RS16440 (U722_17475) - 3427164..3427646 (-) 483 WP_024085863.1 flagellar protein FlgN -
  U722_RS16445 (U722_17480) flgM 3427661..3427927 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  U722_RS16450 (U722_17485) - 3427997..3428416 (-) 420 WP_003151392.1 TIGR03826 family flagellar region protein -
  U722_RS16455 (U722_17490) comFC 3428490..3429179 (-) 690 WP_196616643.1 phosphoribosyltransferase family protein Machinery gene
  U722_RS16460 (U722_17495) - 3429185..3429469 (-) 285 WP_003151389.1 late competence development ComFB family protein -
  U722_RS16465 (U722_17500) comFA 3429527..3430912 (-) 1386 WP_024085865.1 DEAD/DEAH box helicase Machinery gene
  U722_RS16470 (U722_17505) - 3431020..3431868 (-) 849 WP_024085866.1 DegV family protein -
  U722_RS16475 degU 3431966..3432655 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  U722_RS16480 (U722_17515) degS 3432732..3433895 (-) 1164 WP_003151385.1 two-component sensor histidine kinase DegS Regulator
  U722_RS16485 (U722_17520) - 3434118..3434765 (+) 648 WP_003151384.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52491.85 Da        Isoelectric Point: 10.1310

>NTDB_id=64224 U722_RS16465 WP_024085865.1 3429527..3430912(-) (comFA) [Bacillus amyloliquefaciens LFB112]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRINRFIEKEHITAEPSIIRRKNRYVCQRCGQSDQACFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQAPENSWEPVKLSWEGTLTDGQKQAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNQGLRVCVATPRTDVVLELLPRLKKAFEKVDVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKNAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVLFHFGMTNGMKKAKKHIELMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=64224 U722_RS16465 WP_024085865.1 3429527..3430912(-) (comFA) [Bacillus amyloliquefaciens LFB112]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCAGCGCATCAACCGTTTTATAGAAAAAGAGCATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCAGGCCGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCTGGTATCGAGTTT
GCCCTGAATCAAGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGACGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATCTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGGTTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCTGA
CACATTAAAAAAGAATGCTGAAGCAGGTTTGCTGAAAAGCGTTCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGATTCAGAGACG
GCCGGCTTGATCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCCTGTTTCATTTCGGAATGACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACTTATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(328-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.826

99.783

0.627

Multiple sequence alignment