Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LXE94_RS12465 Genome accession   NZ_CP090125
Coordinates   2337471..2338541 (-) Length   356 a.a.
NCBI ID   WP_024573393.1    Uniprot ID   A0A165AJM7
Organism   Bacillus subtilis strain Bs96     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2332471..2343541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXE94_RS12420 (LXE94_12395) yqzG 2332604..2332930 (+) 327 WP_026113671.1 YqzG/YhdC family protein -
  LXE94_RS12425 (LXE94_12400) - 2333047..2334294 (+) 1248 WP_031600262.1 IS256-like element ISBsu2 family transposase -
  LXE94_RS12430 (LXE94_12405) spoIITA 2334350..2334529 (-) 180 WP_014480252.1 YqzE family protein -
  LXE94_RS12435 (LXE94_12410) comGG 2334601..2334975 (-) 375 WP_144453292.1 ComG operon protein ComGG Machinery gene
  LXE94_RS12440 (LXE94_12415) comGF 2334976..2335359 (-) 384 WP_041334965.1 ComG operon protein ComGF Machinery gene
  LXE94_RS12445 (LXE94_12420) comGE 2335385..2335732 (-) 348 WP_041334967.1 ComG operon protein 5 Machinery gene
  LXE94_RS12450 (LXE94_12425) comGD 2335716..2336147 (-) 432 WP_041334970.1 comG operon protein ComGD Machinery gene
  LXE94_RS12455 (LXE94_12430) comGC 2336137..2336433 (-) 297 WP_041334973.1 comG operon protein ComGC Machinery gene
  LXE94_RS12460 (LXE94_12435) comGB 2336447..2337484 (-) 1038 WP_041334977.1 comG operon protein ComGB Machinery gene
  LXE94_RS12465 (LXE94_12440) comGA 2337471..2338541 (-) 1071 WP_024573393.1 competence protein ComGA Machinery gene
  LXE94_RS12470 (LXE94_12445) - 2338689..2338871 (-) 183 WP_257986068.1 hypothetical protein -
  LXE94_RS12475 (LXE94_12450) corA 2338951..2339903 (-) 953 Protein_2410 magnesium transporter CorA -
  LXE94_RS12480 (LXE94_12455) yqhB 2340046..2341374 (+) 1329 WP_237983311.1 hemolysin family protein -
  LXE94_RS12485 (LXE94_12460) rsbRD 2341427..2342263 (-) 837 WP_121548936.1 RsbT co-antagonist protein RsbRD -
  LXE94_RS12495 (LXE94_12470) mgsR 2342486..2342866 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  LXE94_RS12500 (LXE94_12475) yqgY 2343098..2343343 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40472.67 Da        Isoelectric Point: 9.0175

>NTDB_id=640706 LXE94_RS12465 WP_024573393.1 2337471..2338541(-) (comGA) [Bacillus subtilis strain Bs96]
MDSIEKLSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=640706 LXE94_RS12465 WP_024573393.1 2337471..2338541(-) (comGA) [Bacillus subtilis strain Bs96]
TTGGATTCAATAGAAAAGTTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACACTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCTCAATACAATATCCCTTCGATTGATAAACTGTCGTTATTTCCAAAGACAGGAGCCACATTACTCTCATTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAC
GCAAAAAAACACTTTAATCGAAATATTGTTACATTAGAGGACCCTGTTGAAACTAGGGACGAAGACGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAGGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGATGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTGACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGGCTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACCGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGTCCATTTTGTGAAAACGGATGTTCATCAGTATATTGCCGTCAGTCAC
GAAATACCAGAAGAGCTAGCGTTTATGAGCTTCTTTATGGGAAAAACCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A165AJM7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997
  pilB Glaesserella parasuis strain SC1401

43.878

82.584

0.362