Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OEG87_RS19510 Genome accession   NZ_CP106924
Coordinates   3972506..3972946 (+) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain MFDS1016424     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3967506..3977946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OEG87_RS19490 (OEG87_19490) aroP 3968504..3969877 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  OEG87_RS19495 (OEG87_19495) ampE 3969920..3970774 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  OEG87_RS19500 (OEG87_19500) ampD 3970771..3971322 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OEG87_RS19505 (OEG87_19505) nadC 3971410..3972303 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OEG87_RS19510 (OEG87_19510) pilA 3972506..3972946 (+) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  OEG87_RS19515 (OEG87_19515) pilB 3972956..3974341 (+) 1386 WP_001025154.1 type II secretion system protein GspE Machinery gene
  OEG87_RS19520 (OEG87_19520) hofC 3974331..3975533 (+) 1203 WP_000157234.1 protein transport protein HofC -
  OEG87_RS19525 (OEG87_19525) guaC 3975568..3976611 (-) 1044 WP_001217338.1 GMP reductase -
  OEG87_RS19530 (OEG87_19530) - 3976767..3976811 (-) 45 WP_120795372.1 protein YacM -
  OEG87_RS19535 (OEG87_19535) coaE 3976836..3977456 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=638730 OEG87_RS19510 WP_000360902.1 3972506..3972946(+) (pilA) [Escherichia coli strain MFDS1016424]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=638730 OEG87_RS19510 WP_000360902.1 3972506..3972946(+) (pilA) [Escherichia coli strain MFDS1016424]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377