Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LRS65_RS20900 Genome accession   NZ_CP089601
Coordinates   4091825..4092868 (-) Length   347 a.a.
NCBI ID   WP_001013170.1    Uniprot ID   A0A9Q5QS48
Organism   Bacillus cereus strain C-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4086825..4097868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRS65_RS20855 (LRS65_20825) - 4087271..4087471 (-) 201 WP_000106083.1 YqzE family protein -
  LRS65_RS20860 (LRS65_20830) - 4087510..4088007 (-) 498 WP_000836659.1 shikimate kinase -
  LRS65_RS20865 (LRS65_20835) - 4088122..4088772 (-) 651 WP_000183863.1 2OG-Fe(II) oxygenase -
  LRS65_RS20870 (LRS65_20840) comGG 4088947..4089318 (-) 372 WP_025687689.1 competence type IV pilus minor pilin ComGG -
  LRS65_RS20875 (LRS65_20845) comGF 4089315..4089785 (-) 471 WP_000923101.1 competence type IV pilus minor pilin ComGF -
  LRS65_RS20880 (LRS65_20850) - 4089755..4090057 (-) 303 WP_025687688.1 hypothetical protein -
  LRS65_RS20885 (LRS65_20855) comGD 4090050..4090505 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  LRS65_RS20890 (LRS65_20860) comGC 4090502..4090801 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  LRS65_RS20895 (LRS65_20865) comGB 4090813..4091832 (-) 1020 WP_025687687.1 competence type IV pilus assembly protein ComGB -
  LRS65_RS20900 (LRS65_20870) comGA 4091825..4092868 (-) 1044 WP_001013170.1 competence type IV pilus ATPase ComGA Machinery gene
  LRS65_RS20905 (LRS65_20875) - 4093074..4093769 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  LRS65_RS20910 (LRS65_20880) - 4093895..4094137 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  LRS65_RS20915 (LRS65_20885) - 4094245..4095639 (+) 1395 Protein_4081 L-cystine transporter -
  LRS65_RS20920 (LRS65_20890) - 4095718..4096110 (-) 393 WP_000875394.1 hypothetical protein -
  LRS65_RS20925 (LRS65_20895) - 4096198..4096413 (-) 216 WP_001008327.1 DUF3912 family protein -
  LRS65_RS20930 (LRS65_20900) - 4096666..4096977 (+) 312 WP_001093237.1 hypothetical protein -
  LRS65_RS20935 (LRS65_20905) - 4097012..4097500 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39095.50 Da        Isoelectric Point: 8.7212

>NTDB_id=638317 LRS65_RS20900 WP_001013170.1 4091825..4092868(-) (comGA) [Bacillus cereus strain C-1]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAGGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=638317 LRS65_RS20900 WP_001013170.1 4091825..4092868(-) (comGA) [Bacillus cereus strain C-1]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCAGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATATATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGGTGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

40.062

92.795

0.372