Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   LUS72_RS05735 Genome accession   NZ_CP089518
Coordinates   1118955..1122680 (+) Length   1241 a.a.
NCBI ID   WP_097829996.1    Uniprot ID   -
Organism   Bacillus cereus strain PT1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1113955..1127680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUS72_RS05720 - 1114112..1114705 (+) 594 WP_097829993.1 TVP38/TMEM64 family protein -
  LUS72_RS05725 lepB 1114762..1115325 (+) 564 WP_097829994.1 signal peptidase I -
  LUS72_RS05730 addB 1115443..1118958 (+) 3516 WP_264448697.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  LUS72_RS05735 addA 1118955..1122680 (+) 3726 WP_097829996.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  LUS72_RS05740 - 1122704..1122982 (+) 279 WP_097829997.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  LUS72_RS05745 - 1123200..1123907 (+) 708 WP_097829998.1 DNA alkylation repair protein -
  LUS72_RS05750 gerPF 1123964..1124179 (-) 216 WP_002170786.1 spore germination protein GerPF -
  LUS72_RS05755 - 1124222..1124608 (-) 387 WP_097829999.1 spore germination protein GerPE -
  LUS72_RS05760 gerPD 1124622..1124816 (-) 195 WP_001052805.1 spore germination protein GerPD -
  LUS72_RS05765 gerPC 1124823..1125437 (-) 615 WP_097830000.1 spore germination protein GerPC -
  LUS72_RS05770 gerPB 1125512..1125709 (-) 198 WP_097830001.1 spore germination protein GerPB -
  LUS72_RS05775 gerPA 1125724..1125945 (-) 222 WP_001111191.1 spore germination protein GerPA -
  LUS72_RS05780 - 1126042..1126221 (-) 180 WP_002087032.1 aspartyl-phosphate phosphatase Spo0E family protein -
  LUS72_RS05785 - 1126467..1127366 (+) 900 WP_170961003.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142705.01 Da        Isoelectric Point: 4.8696

>NTDB_id=637869 LUS72_RS05735 WP_097829996.1 1118955..1122680(+) (addA) [Bacillus cereus strain PT1]
MIENWPEKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGMLSSAARGSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVLDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRQVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPVDIYEYDTSWK
VEVVDGKDLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLMWEYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
GLGKRVVAAKSVEREVPFTMMLSAEEAYQDWQGKKGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLKHPVKEKCLYFFDGNHVVKVEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=637869 LUS72_RS05735 WP_097829996.1 1118955..1122680(+) (addA) [Bacillus cereus strain PT1]
ATGATAGAAAATTGGCCTGAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAATGGACG
TGATATTTTAGTCGCAGCAGCAGCTGGATCTGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAAATGAAAAATCGAATT
GGAGAAGCGTTAGAAAAAGTATTAATTGATGGGCCAGGTTCACAGCATATAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCCATTTCAACAATCCATTCATTTTGTTTACAAGTGATTAGAGGATACTACTACATGCTTGATGTCGATCCTCGTTTCC
GTATTGCGAACCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACCTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAACCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATACGATGTTGAAGGAA
AGACGATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTGGAAACAGCGGAACAGCATATT
CGTAAAGCGACTGAACTCGCAATGCTTCCTGACGGTCCGGCGCCTCGCGTTGAAACGTTACAAGCGGATTTAGCTTTACT
TGGAATGTTATCCTCAGCAGCTCGTGGGTCGTGGACAAGCGTTTATGAAGCGATGCAAAACGTATCGTGGCAAACGCTAA
AGCGTATTAAGAAAAGTGATTACAACGAAGATGTTGTAAAACAAGTAGATTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAACTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATTAAGCGTGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGCGAGCAAAGTGAAGACGGTGAAATGAAGCCTTCAGCAGTAGCGCTGCAA
TATCGTAATAAATTCGCTGAAGTACTAGTCGATGAATATCAAGATACGAATTTCGTACAAGAATCCATTATTAAATTCGT
AACGAAAGACTCTGAGAGTGAAGGAAACTTGTTCATGGTAGGTGACGTGAAGCAGTCAATTTATCGTTTCCGACTAGCAG
AACCAGGACTGTTTTTAGGGAAATACAAACGATTCACACAAGAAGGATCGGGCGGTGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGTTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGACTATGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCTGAATTACTATGCA
TTCAGCAAACAGAAGAAGAAGTGCTAGACGGTGAAGAAGGTGCAGAAGTAGAAAAAGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCAATGGTCGATTCAGGTTATGAAGTGTATGATCGTAAAACTGATAGTATGCGCCAAGTACAATA
CCGTGATTTCGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCGG
TATATGCTGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATCGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTTGGACTAAATGACGAAGAACTTGCAACGCTTCG
TGCTCATGGAAAGAAAGGTTCATTTTATGAAGTGATGAGTTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAACAAGAAC
TGCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGGTGGCGTGAATTTGCGCGTCAACAATCACTTTCCGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGCTATTACGACTTTGTCGGCGGTTTACCAGCCGGAAAGCAAAGACAGGCAAACTT
ACGCGTACTATATGACCGCGCGAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAGC
GTATTTTAGAACGCGGAGATGATATGGGAACGGCGAGAGCTCTTGGAGAACAAGAAGACGTTGTTCGTATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTCGTATTTGTAGCTGGACTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
ACGCTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CACAACTAGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCAAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCCAATAAGGAAATGGAAAAATGGCTCGATGCGCGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGCTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAATATTCCAGTCGACATTTATGAGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGATGGAAAAGATTTACTTGCACCAGAACCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCGCTAGAAAGTGAACGGAAAGAAGAAGTGTACGATAGATTAATGTGGGAATACGGGTATGAGG
ACGCGACATCGCACCGTGCGAAACAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTTCGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGACTAACGTACGCAGAGCGAGG
GACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAACAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAAGTCATTTCATTCTTTGATAGT
GGTTTAGGTAAAAGAGTAGTAGCAGCAAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGAAGAAAGGCGAATCAATACTTGTCCAAGGGGTTATCGACTGTATGATCGAAGAGGAAGACG
GAATTACATTAATCGACTTTAAAACAGATACGATTGAAGGAAAGTTTCCAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGACCGATACAAAGTACAGCTTTCACTATATGCAAAAGCACTCGAGAAAAGCTTAAAACATCCTGTGAAAGAGAAGTG
TTTATACTTCTTTGATGGGAATCATGTTGTAAAGGTTGAGGAATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1232)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.569

100

0.538