Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   LUS72_RS05730 Genome accession   NZ_CP089518
Coordinates   1115443..1118958 (+) Length   1171 a.a.
NCBI ID   WP_264448697.1    Uniprot ID   -
Organism   Bacillus cereus strain PT1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1110443..1123958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUS72_RS05695 - 1110752..1111246 (-) 495 WP_097829988.1 DUF6584 family protein -
  LUS72_RS05700 cspA 1111524..1111727 (+) 204 WP_000301522.1 RNA chaperone/antiterminator CspA -
  LUS72_RS05705 - 1111921..1112106 (-) 186 WP_097829989.1 hypothetical protein -
  LUS72_RS05710 - 1112371..1112952 (+) 582 WP_097829990.1 competence protein ComK -
  LUS72_RS05715 - 1113195..1113791 (+) 597 WP_097829991.1 DUF4306 domain-containing protein -
  LUS72_RS05720 - 1114112..1114705 (+) 594 WP_097829993.1 TVP38/TMEM64 family protein -
  LUS72_RS05725 lepB 1114762..1115325 (+) 564 WP_097829994.1 signal peptidase I -
  LUS72_RS05730 addB 1115443..1118958 (+) 3516 WP_264448697.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  LUS72_RS05735 addA 1118955..1122680 (+) 3726 WP_097829996.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  LUS72_RS05740 - 1122704..1122982 (+) 279 WP_097829997.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  LUS72_RS05745 - 1123200..1123907 (+) 708 WP_097829998.1 DNA alkylation repair protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134188.17 Da        Isoelectric Point: 5.0622

>NTDB_id=637868 LUS72_RS05730 WP_264448697.1 1115443..1118958(+) (addB) [Bacillus cereus strain PT1]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEEVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLSESEYVKGAEIYIDGFHSFSPQELEIVRGLMRLGTRITITLTIDE
KTLAQPVNELDLFYETTLTYERIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDASEMITDSEREMEEKINQLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRVRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNIKATFVIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKESFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKQYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSTLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFALPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=637868 LUS72_RS05730 WP_264448697.1 1115443..1118958(+) (addB) [Bacillus cereus strain PT1]
ATGTCACTTCGATTTGTGATTGGTAGAGCTGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAACAGGCGTTAATTGGGA
GTGAAGAGGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCGCGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCGGGCGTGCATATGTTACTTCGTAAAATTGTAGAGTCCCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGCAGTAGTGCAGAGCAAAAGCTGCTAGCG
AATAAAGTGTATGATTTACAGTTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAGTATTTAGATTCAGAAGATTA
CTTACAATTATTAATTGAGAAGCTTTCAGAATCTGAATATGTAAAAGGCGCGGAAATTTATATAGATGGATTCCATTCAT
TTTCCCCTCAAGAGCTAGAAATTGTTAGAGGGCTAATGAGATTAGGAACTAGAATCACAATCACATTGACAATAGATGAA
AAAACGTTAGCGCAGCCAGTGAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAACGAATAAAGCAAGTAGC
TCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCGTTGATGGAGCAGCCGCGTTTTCATTCTCCGGCATTAGCTCATT
TAGAAGCCCATTATGAAGCGCGTCCGAATGAAAAATTTAACGGTGAAGCAAGTGTAACGATTAGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTTGCTCGTGAAATTAGAAGACTTGTGGCGGATGAAGACTATCGTTACCGAGATATCGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGACGTAATGCGAACGTTATTTACAGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCAATGTCACATCATCCGCTAGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTATTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGATGTAAGAAAAGAAGCGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCAGAAGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGATGATGCGAGCGAAATGATAACAGACAGTGAGCGTGAAATGGAAGAGAAAATAAATCAACTTCGTGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGGCTGAAGCGTGCTGGAACAGTTATGCAAATGTGCGAAGCGGTATA
TTTATTTTTAGAGGAGCTTGACGTTCCGAAAAAACTAGAAGAATTACGTGTTCGTGCAGAAGAAAGTGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTGATGAGCCTTCTTGATACGTTTGTAGAGATGCTTGGTGAGGAGAAG
ATGTCACTATCTATGTTCACGGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTTGCGAACATTCCGCCGTCACTAGA
TCAAGTGTTAATTGCTAATATTGATCGTTCTAGATTATCAAATATTAAAGCGACATTCGTTATTGGAGTGAATGAAGGCG
TTATTCCAGCAGCACCTATGGATGAAGGGATGCTGTCTGATGAGGAAAGAGAAGTTCTTGGCGCGGCAGGAATTGAATTA
GCGCCAACGACGAGACAAACTTTATTAGAAGAACAGTTTGTTATGTATCAAATGGTAACGAGAGCATCTGAGAAATTATA
CATTTCATGCCCGCTTGCAGATGAAGAAGGGAAGACGTTACTTGCTTCTAGTTTTATTAAAAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAATCATTTATTACAAATGACGTAAATGACTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTGACATTGTCTTATGTCATGCAGCAGCTGCAAACATGGAAGCAATACGGATTTGAAGGTAATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTTACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTGTTATCAAGTTTATTCTACCGAA
ATCGTGCACAGAAACTAAGTACAGCGGTAAGTAGAGATTTATACGGAGATACAATTAAGGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTACGCTCATTTCGCACAGCACGGTTTATCGCTAAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATCGGTGAACTATTCCATGCAGCGCTGAAAAGAATTGCAGACAGGTTATTACGTGAAAACCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTACTTTAGTAATAGAAGAAATTGCACCATTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATATTCCGTACATCACTCATTCTTCGTGAACA
TGCGAAGTCTAGCGGTTTTGTACCAGTTGATTTAGAAGTACCGTTTGGTATGGGCGGGACTGGATCTCTTCCGCCAATGG
AATTTGCGTTGCCAAATGGTGTGAAGATGGAAGTAGTGGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAGAGTGGT
ACGTTCCTTCGTATTATTGACTATAAATCAAGTTCGAAAGCGTTAGATTTAACAGAAGTGTATTACGGACTAGCGCTTCA
AATGTTAACGTATTTGGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGCGGCACTGCATCACCGGCGGGTG
TATTGTATTTCCATATTCATAACCCGATTGTTGAGATGAAAGGCGATGCATCTGAAGAAGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGGCTCGTACTAGGAGATGCTGATGTTGTTCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGATGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAACAAGAGTTTA
ATGTACTTCAAAAATATGTACACCACTCGTTTGAAAATATCGGAAAAGACATCACAGAAGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCTTGTACGTTCTGTAACTTTAAATCTGTTTGTCAGTTTGATGAATCACTTGAAGATAA
CCAATTCCGTACGTTAAAAGATATGAAAGATAGCGAAGCAATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.317

100

0.494