Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   LUZ08_RS10295 Genome accession   NZ_CP089489
Coordinates   2056724..2058352 (+) Length   542 a.a.
NCBI ID   WP_001186596.1    Uniprot ID   -
Organism   Staphylococcus aureus strain E1174_IV_ST22     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 2055357..2110961 2056724..2058352 within 0


Gene organization within MGE regions


Location: 2055357..2110961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUZ08_RS10290 ccrA 2055357..2056703 (+) 1347 WP_000816499.1 cassette chromosome recombinase CcrA Machinery gene
  LUZ08_RS10295 ccrB 2056724..2058352 (+) 1629 WP_001186596.1 cassette chromosome recombinase CcrB Machinery gene
  LUZ08_RS10300 - 2058823..2059173 (+) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  LUZ08_RS10305 - 2059258..2059569 (+) 312 WP_000833212.1 DUF960 family protein -
  LUZ08_RS10310 - 2059588..2060109 (+) 522 WP_000836008.1 DUF1643 domain-containing protein -
  LUZ08_RS10315 - 2060111..2060317 (+) 207 WP_001077283.1 hypothetical protein -
  LUZ08_RS10320 - 2060310..2060633 (+) 324 WP_000088091.1 JAB domain-containing protein -
  LUZ08_RS10325 - 2060828..2062720 (+) 1893 WP_002485298.1 site-specific integrase -
  LUZ08_RS10330 - 2062727..2063104 (+) 378 WP_001041908.1 DUF6262 family protein -
  LUZ08_RS10335 - 2063342..2063707 (+) 366 WP_000159127.1 metalloregulator ArsR/SmtB family transcription factor -
  LUZ08_RS10340 - 2063700..2066114 (+) 2415 WP_000378487.1 cadmium-translocating P-type ATPase CadA -
  LUZ08_RS10345 - 2066195..2066812 (+) 618 WP_000562107.1 CadD family cadmium resistance transporter -
  LUZ08_RS10350 - 2067086..2067724 (-) 639 Protein_2005 sulfite exporter TauE/SafE family protein -
  LUZ08_RS10355 cstR 2067724..2067984 (-) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  LUZ08_RS10360 - 2068120..2069184 (+) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  LUZ08_RS10365 cstB 2069217..2070551 (+) 1335 Protein_2008 persulfide dioxygenase-sulfurtransferase CstB -
  LUZ08_RS10370 - 2070665..2071774 (-) 1110 WP_000943131.1 ROK family transcriptional regulator -
  LUZ08_RS10375 psm-mec 2072133..2072201 (+) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  LUZ08_RS10380 mecI 2072286..2072657 (-) 372 Protein_2011 mecA-type methicillin resistance repressor MecI -
  LUZ08_RS10385 - 2072657..2074248 (-) 1592 Protein_2012 BlaR1 family beta-lactam sensor/signal transducer -
  LUZ08_RS10390 mecA 2074345..2076354 (+) 2010 WP_078056515.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  LUZ08_RS10395 - 2076400..2076828 (-) 429 WP_000872606.1 MaoC family dehydratase -
  LUZ08_RS10400 - 2076925..2077668 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  LUZ08_RS10405 - 2078545..2078934 (-) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  LUZ08_RS10410 - 2078970..2079644 (+) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  LUZ08_RS10415 - 2079733..2080687 (-) 955 Protein_2018 replication initiation factor domain-containing protein -
  LUZ08_RS10420 mobV 2081214..2082456 (-) 1243 Protein_2019 MobV family relaxase -
  LUZ08_RS10425 tet(K) 2082642..2084021 (-) 1380 WP_000492283.1 tetracycline efflux MFS transporter Tet(K) -
  LUZ08_RS10430 - 2084218..2084892 (+) 675 WP_001106055.1 IS6-like element IS257 family transposase -
  LUZ08_RS10435 - 2085024..2085266 (+) 243 WP_001803415.1 LytTR family DNA-binding domain-containing protein -
  LUZ08_RS10440 - 2085337..2085627 (+) 291 WP_169301211.1 DUF3021 domain-containing protein -
  LUZ08_RS10445 - 2086060..2086734 (+) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  LUZ08_RS10450 - 2086770..2087300 (+) 531 WP_041205518.1 hypothetical protein -
  LUZ08_RS10455 merB 2087495..2088145 (-) 651 WP_000790333.1 organomercurial lyase MerB -
  LUZ08_RS10460 merA 2088227..2089870 (-) 1644 WP_000193219.1 mercury(II) reductase -
  LUZ08_RS10465 - 2089928..2090266 (-) 339 WP_000968575.1 hypothetical protein -
  LUZ08_RS10470 - 2090401..2091081 (-) 681 WP_033842288.1 cytochrome c biogenesis CcdA family protein -
  LUZ08_RS10475 - 2091078..2091431 (-) 354 WP_033842287.1 TlpA disulfide reductase family protein -
  LUZ08_RS10480 merR 2091448..2091855 (-) 408 WP_000425271.1 Hg(II)-responsive transcriptional regulator MerR -
  LUZ08_RS10485 - 2092155..2093501 (-) 1347 WP_000163428.1 NAD(P)/FAD-dependent oxidoreductase -
  LUZ08_RS10490 - 2093784..2094458 (+) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  LUZ08_RS10495 - 2094520..2096733 (+) 2214 Protein_2034 type I restriction endonuclease subunit R -
  LUZ08_RS10500 - 2096811..2097134 (-) 324 WP_001176049.1 PepSY domain-containing protein -
  LUZ08_RS10505 - 2097528..2097749 (-) 222 WP_002504703.1 hypothetical protein -
  LUZ08_RS10510 - 2097698..2098141 (-) 444 WP_000941333.1 hypothetical protein -
  LUZ08_RS10515 - 2098203..2098730 (-) 528 WP_000717929.1 hypothetical protein -
  LUZ08_RS10520 - 2098865..2099353 (-) 489 WP_000393586.1 hypothetical protein -
  LUZ08_RS10525 - 2099369..2099659 (-) 291 WP_000065261.1 hypothetical protein -
  LUZ08_RS10530 - 2099716..2100588 (-) 873 WP_000373149.1 HTH domain-containing protein -
  LUZ08_RS10535 - 2100758..2100904 (-) 147 Protein_2042 JAB domain-containing protein -
  LUZ08_RS10540 - 2101136..2101798 (-) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  LUZ08_RS10545 - 2102190..2102249 (+) 60 WP_181875734.1 erythromycin resistance leader peptide -
  LUZ08_RS10550 erm(A) 2102307..2103038 (+) 732 WP_001072197.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  LUZ08_RS10555 - 2103164..2103946 (-) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  LUZ08_RS10560 - 2104097..2104474 (-) 378 WP_000361059.1 DUF6262 family protein -
  LUZ08_RS10565 - 2104481..2106373 (-) 1893 WP_001557544.1 site-specific integrase -
  LUZ08_RS10570 - 2106370..2107455 (-) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  LUZ08_RS10575 - 2107574..2107891 (-) 318 WP_130825069.1 JAB domain-containing protein -
  LUZ08_RS10580 - 2107912..2108418 (-) 507 WP_000849085.1 DUF1643 domain-containing protein -
  LUZ08_RS10585 - 2108434..2108745 (-) 312 WP_001083108.1 DUF960 family protein -
  LUZ08_RS10590 - 2108841..2109179 (-) 339 WP_000171557.1 SAUGI family uracil-DNA glycosylase inhibitor -
  LUZ08_RS10595 ccrC 2109285..2110961 (-) 1677 WP_000676597.1 cassette chromosome recombinase CcrC -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62274.15 Da        Isoelectric Point: 9.7334

>NTDB_id=637404 LUZ08_RS10295 WP_001186596.1 2056724..2058352(+) (ccrB) [Staphylococcus aureus strain E1174_IV_ST22]
MQQLKQKRVGIYVRVSTEIQSTEGYSIDGQINQIREYCDFNNFVVVDVYADRGISGKSMNRPELQRLLKDANEGQIDSVM
VYKTNRLARNTSDLLKIVEDLHRQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSIGSVTYILSNPFYVGKIQFAK
YKDWNEKRRKGLNDKPIIAEGKHSPIIIQDLWDKVQLRKKQVSQKPQVHGKGTNLLTGIVHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEKYVMDQILEIVKSDKVINQVLERVNQENKVDIGALNHDIAYKQQQYD
EVSGKLHNLVKTIEDNPDLTSALKATIHQYETQLNDITNQMNQLKQQQNQEKLSYDTKQIAALLQRIFQNIESMDKAQLK
ALYLTVIDRIDIRKDGNHKKQFYVTLKLNNEIIKQLFNNTPLDEVLLSTSSLFLPQTLFLQI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=637404 LUZ08_RS10295 WP_001186596.1 2056724..2058352(+) (ccrB) [Staphylococcus aureus strain E1174_IV_ST22]
ATGCAACAACTCAAACAAAAACGTGTCGGTATCTATGTTCGTGTATCAACGGAAATCCAAAGTACTGAAGGCTATAGTAT
CGATGGACAAATCAATCAAATTCGAGAATATTGTGATTTCAATAACTTTGTTGTTGTAGATGTATACGCGGATAGAGGTA
TCTCTGGAAAATCTATGAACCGACCAGAACTACAACGTTTGTTAAAAGATGCGAACGAAGGTCAGATTGATTCTGTTATG
GTCTACAAAACAAACCGACTAGCACGTAACACTTCTGACTTACTCAAAATTGTTGAAGACCTTCATCGTCAAAATGTCGA
ATTCTTCAGCTTATCTGAGCGTATGGAAGTCAATACAAGCAGTGGTAAATTGATGCTACAAATTCTAGCGAGTTTTTCAG
AATTTGAAAGAAATAATATTGTCGAAAATGTATTCATGGGTCAAACCCGACGCGCTCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCGGATAGCAAGCATGAACTCATGATAAACCAACATGAAGCGAATATTGTCAAATA
TATATTTGAGTCATATGCTAAAGGCCACGGATATCGTAAAATTGCGAATGCACTCAATCACAAAGGATACGTGACTAAAA
AAGGAAAGCCTTTCAGTATTGGTTCAGTGACCTATATCTTATCTAATCCATTCTATGTTGGTAAAATTCAATTCGCAAAG
TACAAAGATTGGAATGAAAAGCGTCGTAAAGGGCTGAATGATAAACCAATAATAGCTGAAGGTAAGCATTCCCCTATTAT
TATTCAAGACTTATGGGATAAAGTCCAATTACGTAAAAAACAAGTCAGTCAAAAACCTCAAGTCCACGGTAAAGGAACTA
ATCTATTAACAGGTATCGTTCATTGTCCACAATGTGGTGCACCAATGGCAGCTAGTAACACAACGAACACATTGAAAGAT
GGTACCAAGAAGCGAATACGTTATTATTCTTGCAGTAACTTCCGAAACAAAGGCTCAAAAGTATGTTCTGCGAATAGCGT
TAGAGCTGATGTGATTGAGAAATACGTCATGGATCAAATACTCGAAATTGTCAAAAGTGATAAAGTCATTAACCAAGTCT
TAGAACGTGTCAATCAAGAAAATAAAGTCGATATTGGTGCATTGAACCACGATATCGCTTATAAACAACAACAATACGAT
GAAGTCAGCGGGAAACTCCATAATTTAGTTAAAACCATTGAAGATAATCCGGACCTAACATCTGCATTGAAAGCAACTAT
TCATCAATATGAAACACAACTCAATGACATTACAAATCAAATGAATCAACTCAAACAGCAACAAAATCAAGAGAAACTAT
CTTATGATACGAAACAAATCGCTGCCCTATTACAACGAATATTTCAAAATATAGAATCAATGGATAAAGCACAACTCAAA
GCATTATATCTTACAGTCATTGACCGTATTGATATTCGTAAAGACGGTAATCATAAAAAACAGTTCTACGTTACACTAAA
ACTCAATAATGAAATTATTAAACAACTTTTCAATAATACCCCTCTCGACGAAGTGCTCCTCAGCACTTCGTCTTTATTTT
TGCCTCAAACGCTCTTTCTTCAAATCTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

86.531

100

0.865