Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   YBT1518_RS23020 Genome accession   NC_022873
Coordinates   4587800..4588843 (-) Length   347 a.a.
NCBI ID   WP_001013177.1    Uniprot ID   -
Organism   Bacillus thuringiensis YBT-1518     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4582800..4593843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  YBT1518_RS22975 (YBT1518_23370) - 4583234..4583434 (-) 201 WP_023523009.1 YqzE family protein -
  YBT1518_RS22980 (YBT1518_23375) aroK 4583473..4583970 (-) 498 WP_000836649.1 shikimate kinase AroK -
  YBT1518_RS22985 (YBT1518_23380) - 4584086..4584736 (-) 651 WP_023523010.1 2OG-Fe(II) oxygenase -
  YBT1518_RS22990 (YBT1518_23385) comGG 4584910..4585281 (-) 372 WP_023523011.1 competence type IV pilus minor pilin ComGG -
  YBT1518_RS22995 (YBT1518_23390) comGF 4585278..4585748 (-) 471 WP_000923097.1 competence type IV pilus minor pilin ComGF -
  YBT1518_RS23000 (YBT1518_23395) - 4585718..4586020 (-) 303 WP_000829462.1 hypothetical protein -
  YBT1518_RS23005 (YBT1518_23400) comGD 4586013..4586468 (-) 456 WP_002094140.1 competence type IV pilus minor pilin ComGD -
  YBT1518_RS23010 (YBT1518_23405) comGC 4586465..4586764 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  YBT1518_RS23015 (YBT1518_23410) comGB 4586776..4587807 (-) 1032 WP_023523013.1 competence type IV pilus assembly protein ComGB -
  YBT1518_RS23020 (YBT1518_23415) comGA 4587800..4588843 (-) 1044 WP_001013177.1 competence type IV pilus ATPase ComGA Machinery gene
  YBT1518_RS23025 (YBT1518_23420) - 4589049..4589744 (+) 696 WP_000434124.1 metalloregulator ArsR/SmtB family transcription factor -
  YBT1518_RS23030 (YBT1518_23425) - 4589871..4590113 (+) 243 WP_023523014.1 DUF2626 domain-containing protein -
  YBT1518_RS23035 (YBT1518_23430) - 4590221..4591615 (+) 1395 WP_023523015.1 L-cystine transporter -
  YBT1518_RS38365 - 4591695..4592087 (-) 393 Protein_4616 hypothetical protein -
  YBT1518_RS23045 (YBT1518_23445) - 4592175..4592390 (-) 216 WP_001008326.1 DUF3912 family protein -
  YBT1518_RS23050 (YBT1518_23450) - 4592644..4592955 (+) 312 WP_001093240.1 hypothetical protein -
  YBT1518_RS23055 (YBT1518_23455) - 4592991..4593479 (-) 489 WP_000764263.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39177.60 Da        Isoelectric Point: 8.8944

>NTDB_id=63633 YBT1518_RS23020 WP_001013177.1 4587800..4588843(-) (comGA) [Bacillus thuringiensis YBT-1518]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRHCIKKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=63633 YBT1518_RS23020 WP_001013177.1 4587800..4588843(-) (comGA) [Bacillus thuringiensis YBT-1518]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACATTGTATTAAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTGCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTCATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATAAGAAAAGGATATGCTTTAGGTTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.196

100

0.562
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment