Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LT232_RS15975 Genome accession   NZ_CP089287
Coordinates   3224975..3226360 (+) Length   461 a.a.
NCBI ID   WP_099320008.1    Uniprot ID   -
Organism   Bacillus velezensis strain GSBZ09     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3219975..3231360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LT232_RS15955 (LT232_15955) - 3221121..3221768 (-) 648 WP_007407485.1 YigZ family protein -
  LT232_RS15960 (LT232_15960) degS 3221991..3223154 (+) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  LT232_RS15965 (LT232_15965) degU 3223231..3223920 (+) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  LT232_RS15970 (LT232_15970) - 3224018..3224866 (+) 849 WP_094031329.1 DegV family protein -
  LT232_RS15975 (LT232_15975) comFA 3224975..3226360 (+) 1386 WP_099320008.1 DEAD/DEAH box helicase Machinery gene
  LT232_RS15980 (LT232_15980) - 3226418..3226702 (+) 285 WP_007407481.1 late competence development ComFB family protein -
  LT232_RS15985 (LT232_15985) comFC 3226708..3227397 (+) 690 WP_198161198.1 phosphoribosyltransferase family protein Machinery gene
  LT232_RS15990 (LT232_15990) - 3227471..3227890 (+) 420 WP_025285352.1 TIGR03826 family flagellar region protein -
  LT232_RS15995 (LT232_15995) flgM 3227960..3228226 (+) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  LT232_RS16000 (LT232_16000) - 3228241..3228723 (+) 483 WP_003151394.1 flagellar protein FlgN -
  LT232_RS16005 (LT232_16005) flgK 3228739..3230256 (+) 1518 WP_025285351.1 flagellar hook-associated protein FlgK -
  LT232_RS16010 (LT232_16010) flgL 3230268..3231185 (+) 918 WP_007407477.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52775.27 Da        Isoelectric Point: 10.1560

>NTDB_id=636185 LT232_RS15975 WP_099320008.1 3224975..3226360(+) (comFA) [Bacillus velezensis strain GSBZ09]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYYWNQAYENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNVRAEGVYAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=636185 LT232_RS15975 WP_099320008.1 3224975..3226360(+) (comFA) [Bacillus velezensis strain GSBZ09]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGACCAGCGCATCAAGCGTTTTATAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTATTGGAATCAGGCCTATGAAAACAG
CTGGGAACCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCTCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGTTAATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACCGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACATGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAATGTAAGGGCAGAAGGCGTTTATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(329-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.391

99.783

0.623
  comFA Latilactobacillus sakei subsp. sakei 23K

39.713

90.672

0.36