Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LT232_RS11375 Genome accession   NZ_CP089287
Coordinates   2343619..2344056 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain GSBZ09     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2338619..2349056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LT232_RS11360 (LT232_11360) - 2341477..2341839 (-) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -
  LT232_RS11365 (LT232_11365) hxlA 2342071..2342706 (+) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  LT232_RS11370 (LT232_11370) hxlB 2342703..2343260 (+) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  LT232_RS11375 (LT232_11375) nucA/comI 2343619..2344056 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LT232_RS11380 (LT232_11380) nin/comJ 2344077..2344475 (+) 399 WP_007609299.1 competence protein ComJ Regulator
  LT232_RS11385 (LT232_11385) - 2344516..2345952 (-) 1437 WP_076425807.1 glycoside hydrolase family 1 protein -
  LT232_RS11390 (LT232_11390) - 2346056..2346334 (-) 279 WP_263612330.1 YckD family protein -
  LT232_RS11395 (LT232_11395) - 2346452..2346886 (-) 435 WP_094031491.1 RDD family protein -
  LT232_RS11400 (LT232_11400) - 2346901..2348037 (-) 1137 WP_094031490.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=636167 LT232_RS11375 WP_003156588.1 2343619..2344056(+) (nucA/comI) [Bacillus velezensis strain GSBZ09]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=636167 LT232_RS11375 WP_003156588.1 2343619..2344056(+) (nucA/comI) [Bacillus velezensis strain GSBZ09]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641