Detailed information NTG0063609

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: YBT1518_RS07025
• Genome accession: NC_022873
• Coordinates: 1382085..1385810 (+)
• Length: 1241 a.a.
• NCBI ID: WP_023521414.1
• Uniprot ID: A0A9X7BNI4
• Organism: Bacillus thuringiensis YBT-1518
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1377085..1390810

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
YBT1518_RS07010 (YBT1518_06740)	-	1377243..1377836 (+)	594	WP_023521411.1	TVP38/TMEM64 family protein	-
YBT1518_RS07015 (YBT1518_06745)	lepB	1377893..1378456 (+)	564	WP_023521412.1	signal peptidase I	-
YBT1518_RS07020 (YBT1518_06750)	addB	1378573..1382088 (+)	3516	WP_023521413.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
YBT1518_RS07025 (YBT1518_06755)	addA	1382085..1385810 (+)	3726	WP_023521414.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
YBT1518_RS07030 (YBT1518_06760)	-	1385823..1386110 (+)	288	WP_000255723.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
YBT1518_RS07035 (YBT1518_06765)	gerPF	1386248..1386463 (-)	216	WP_001141570.1	spore germination protein GerPF	-
YBT1518_RS07040 (YBT1518_06770)	-	1386506..1386892 (-)	387	WP_000902329.1	spore germination protein GerPE	-
YBT1518_RS07045 (YBT1518_06775)	gerPD	1386908..1387102 (-)	195	WP_001052807.1	spore germination protein GerPD	-
YBT1518_RS07050 (YBT1518_06780)	gerPC	1387109..1387723 (-)	615	WP_001070761.1	spore germination protein GerPC	-
YBT1518_RS07055 (YBT1518_06785)	gerPB	1387791..1387997 (-)	207	WP_001012508.1	spore germination protein GerPB	-
YBT1518_RS07060 (YBT1518_06790)	gerPA	1388012..1388233 (-)	222	WP_001111187.1	spore germination protein GerPA	-
YBT1518_RS07065	-	1388338..1388505 (-)	168	Protein_1343	aspartyl-phosphate phosphatase Spo0E family protein	-
YBT1518_RS07070 (YBT1518_06795)	-	1388753..1389652 (+)	900	WP_001213080.1	fumarylacetoacetate hydrolase family protein	-
YBT1518_RS07075 (YBT1518_06800)	-	1389687..1389968 (-)	282	WP_000926859.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142720.79 Da
• Isoelectric Point: 4.8002

>NTDB_id=63609 YBT1518_RS07025 WP_023521414.1 1382085..1385810(+) (addA) [Bacillus thuringiensis YBT-1518]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADVVLLGTLSSAARESWTSLYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEDLATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHNEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITAETLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=63609 YBT1518_RS07025 WP_023521414.1 1382085..1385810(+) (addA) [Bacillus thuringiensis YBT-1518]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGGCG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCGCAAGAGATGAAAAACCGAATC
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAACCAGGCTCTCAACACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTTATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGCATCGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCGATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACTCTGCAAGCAGATGTAGTTTTACT
TGGAACGCTATCATCAGCTGCTCGTGAGTCGTGGACAAGTTTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGACAAAGGAATGGTCGACTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCTTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTGTTAGTCGATGAATATCAAGATACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGCGACGTAAAGCAGTCAATTTATCGTTTCCGACTAGCCG
AACCAGGCTTATTCCTAGGAAAGTATAAACGCTTTACGCAAGAAGGATCGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AATTTCCGTAGTCGTCATGAAGTGCTAGCAGGAACGAACTTCATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAGCTATTATGTA
TTCAGCAAACGGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTAGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCTATGGTTGATTCCGGTTACGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGATTTCGTTATTTTACTCCGCTCGATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATATGCTGACCTTGCGACTGGTTATTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATTGTGGGACTAAATGATGAAGACCTTGCGACGCTTCG
TGCTCATGGGAAGAAAGGGTCATTTTATGAAGTAATGAGCTCGTTCTTAAAAGGAGCACCGCTTGAAGAAGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAATTTACTGCAAGGATGGCGTGAATTTGCCCGTCAACAATCCCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCAGGAAAGCAAAGACAAGCAAACTT
ACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGGTTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCCCTAGGTGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
ACGCTTCTTACTGCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCAATTAAACGCAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGTGTATTATATGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAATGAATGGTTATTACCAGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTCCTTGAATTAGGACAAGGAAGCATTCCGGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCGGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGTGG
AACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCGATTACGGCCGAAACGCTACAAGAACAAATCGCAG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCAATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAAAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCGAAACCAATTTTA
GAAGATCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCGCTTGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.168	100	0.534