Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LTK18_RS08725 Genome accession   NZ_CP089165
Coordinates   1715584..1716081 (+) Length   165 a.a.
NCBI ID   WP_029650985.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UNC_SaCF07     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1710584..1721081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTK18_RS08685 (LTK18_08685) - 1710628..1711614 (+) 987 WP_000161311.1 ROK family glucokinase -
  LTK18_RS08690 (LTK18_08690) - 1711614..1711943 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  LTK18_RS08695 (LTK18_08695) - 1711940..1712563 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  LTK18_RS08700 (LTK18_08700) comGA 1712615..1713589 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  LTK18_RS08705 (LTK18_08705) comGB 1713561..1714631 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  LTK18_RS08710 (LTK18_08710) comGC 1714645..1714956 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  LTK18_RS08715 (LTK18_08715) comGD 1714934..1715380 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  LTK18_RS08720 (LTK18_08720) comGE 1715367..1715666 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  LTK18_RS08725 (LTK18_08725) comGF 1715584..1716081 (+) 498 WP_029650985.1 competence type IV pilus minor pilin ComGF Machinery gene
  LTK18_RS08730 (LTK18_08730) - 1716178..1716324 (+) 147 WP_001792109.1 hypothetical protein -
  LTK18_RS08735 (LTK18_08735) - 1716314..1716838 (+) 525 WP_001015120.1 shikimate kinase -
  LTK18_RS08740 (LTK18_08740) gcvT 1716997..1718088 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  LTK18_RS08745 (LTK18_08745) gcvPA 1718108..1719454 (+) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  LTK18_RS08750 (LTK18_08750) gcvPB 1719447..1720919 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.05 Da        Isoelectric Point: 10.3787

>NTDB_id=635622 LTK18_RS08725 WP_029650985.1 1715584..1716081(+) (comGF) [Staphylococcus aureus strain UNC_SaCF07]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEKMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=635622 LTK18_RS08725 WP_029650985.1 1715584..1716081(+) (comGF) [Staphylococcus aureus strain UNC_SaCF07]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAAAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

99.394

100

0.994
  comGF Staphylococcus aureus MW2

95.758

100

0.958