Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LTK15_RS03565 Genome accession   NZ_CP089154
Coordinates   645954..646928 (+) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain UNC_SaCF36     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 640954..651928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTK15_RS03530 (LTK15_03530) rpmG 641414..641563 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  LTK15_RS03535 (LTK15_03535) - 641772..642311 (+) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  LTK15_RS03540 (LTK15_03540) - 642323..643786 (+) 1464 WP_001017870.1 rhomboid family intramembrane serine protease -
  LTK15_RS03545 (LTK15_03545) - 643767..643970 (+) 204 WP_000087561.1 YqgQ family protein -
  LTK15_RS03550 (LTK15_03550) - 643967..644953 (+) 987 WP_000161307.1 ROK family glucokinase -
  LTK15_RS03555 (LTK15_03555) - 644953..645282 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  LTK15_RS03560 (LTK15_03560) - 645279..645902 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  LTK15_RS03565 (LTK15_03565) comGA 645954..646928 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  LTK15_RS03570 (LTK15_03570) comGB 646900..647970 (+) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  LTK15_RS03575 (LTK15_03575) comGC 647984..648295 (+) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  LTK15_RS03580 (LTK15_03580) comGD 648273..648719 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  LTK15_RS03585 (LTK15_03585) comGE 648706..649005 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  LTK15_RS03590 (LTK15_03590) comGF 648923..649420 (+) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  LTK15_RS03595 (LTK15_03595) - 649517..649663 (+) 147 WP_001789879.1 hypothetical protein -
  LTK15_RS03600 (LTK15_03600) - 649653..650177 (+) 525 WP_033858309.1 shikimate kinase -
  LTK15_RS03605 (LTK15_03605) gcvT 650336..651427 (+) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=635359 LTK15_RS03565 WP_000697220.1 645954..646928(+) (comGA) [Staphylococcus aureus strain UNC_SaCF36]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=635359 LTK15_RS03565 WP_000697220.1 645954..646928(+) (comGA) [Staphylococcus aureus strain UNC_SaCF36]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGACTTGATGTATCTACACAACAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTGATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1