Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   LRR78_RS22115 Genome accession   NZ_CP089094
Coordinates   4615684..4617699 (+) Length   671 a.a.
NCBI ID   WP_002742599.1    Uniprot ID   -
Organism   Microcystis aeruginosa FACHB-905 = DIANCHI905     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4610684..4622699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRR78_RS22085 (LRR78_22085) - 4610927..4611322 (-) 396 WP_002738951.1 PH domain-containing protein -
  LRR78_RS22090 (LRR78_22090) rnpA 4611312..4611674 (-) 363 WP_084989793.1 ribonuclease P protein component -
  LRR78_RS22095 (LRR78_22095) rpmH 4611701..4611838 (-) 138 WP_002739276.1 50S ribosomal protein L34 -
  LRR78_RS22100 (LRR78_22100) - 4611930..4613894 (+) 1965 WP_002742602.1 amylo-alpha-1,6-glucosidase -
  LRR78_RS22105 (LRR78_22105) - 4613985..4614626 (+) 642 WP_002742601.1 HAD-IA family hydrolase -
  LRR78_RS22110 (LRR78_22110) grpE 4614721..4615443 (-) 723 WP_002742600.1 nucleotide exchange factor GrpE -
  LRR78_RS22115 (LRR78_22115) pilB/pilB1 4615684..4617699 (+) 2016 WP_002742599.1 GspE/PulE family protein Machinery gene
  LRR78_RS22120 (LRR78_22120) pilT 4617852..4618958 (+) 1107 WP_002742598.1 type IV pilus twitching motility protein PilT Machinery gene
  LRR78_RS22125 (LRR78_22125) - 4619099..4620313 (+) 1215 WP_036400185.1 type II secretion system F family protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75292.54 Da        Isoelectric Point: 6.5258

>NTDB_id=635194 LRR78_RS22115 WP_002742599.1 4615684..4617699(+) (pilB/pilB1) [Microcystis aeruginosa FACHB-905 = DIANCHI905]
MTFSSKKTRAVALRNYFSPFGNKLVQSGYIGAEQMQQALVETRKSGRSLVEILQKLTGRPLPPDLQRQYKKNQLFELKIL
YGVESIDPELSDVDGLEIARLIDSLIPIDLCRRYRLIPLRRIEGEPTAILIAMVDPDNLAATDDINRILRPRELGLQRLV
IAGEDYERLLEKFYQAQSELEREKARLQKEKELEKLSDLTDIVGSLDLTSGVANDEVADDLGEGDANQAPVINLVNKIIA
KALQEGSSDIHIEPQEEFLKIRFRKDGVLHQAFEPLPKKIAPAVTARFKIMADLDISERRLPQDGKIRRMYQGRKVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDRNTLQIFRDLASRPFGLLLVTGPTGSGKSTTLYSVLAERNHPGININTA
EDPIEYSLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTVIEAALTGHLVLTTLHTNDAAGAIVRLD
KMGIEPFMISGSLLGILAQRLVRRVCTQCRVAYHPSQEELARFGLSAANEQEVTFYKANTLTMEEIQQARKQGNLCLKCA
GSGYKDRIGVYEVMQNSERIQQLINQGATTDRIKEAAVDEGMVTLLAYSLNLVREGYTTLEEVERVTLTDSGLEAELKAK
RKSGLICSGCRAELQPEWLDCPYCMRPRFSY

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=635194 LRR78_RS22115 WP_002742599.1 4615684..4617699(+) (pilB/pilB1) [Microcystis aeruginosa FACHB-905 = DIANCHI905]
ATGACATTTTCTTCCAAGAAAACCCGTGCTGTCGCCCTACGCAATTACTTTTCCCCTTTTGGTAACAAGTTAGTGCAGTC
GGGCTATATCGGTGCCGAGCAAATGCAGCAGGCCCTGGTGGAAACGCGGAAATCGGGACGATCGCTAGTAGAAATATTGC
AAAAACTAACCGGGCGGCCTTTGCCCCCGGATTTACAGCGACAGTATAAGAAAAACCAGTTATTTGAACTAAAAATTCTC
TACGGAGTCGAATCGATCGACCCCGAGTTGAGCGACGTGGATGGACTGGAAATAGCCAGGCTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGCGTCGAATCGAAGGAGAGCCAACCGCGATTTTGATAGCCATGG
TCGATCCCGACAACCTCGCGGCTACTGACGATATCAATCGTATTCTCCGCCCCAGGGAGTTAGGTTTACAGCGTTTGGTG
ATTGCCGGGGAAGATTATGAAAGATTACTGGAAAAATTCTACCAAGCCCAATCGGAACTAGAACGGGAGAAAGCCCGACT
ACAAAAAGAAAAAGAACTGGAAAAACTCTCGGATCTGACCGATATTGTCGGCAGTCTTGATTTGACCTCTGGTGTTGCTA
ACGATGAAGTGGCCGATGATCTCGGAGAAGGGGATGCTAATCAAGCCCCCGTCATCAACCTTGTCAATAAAATTATAGCT
AAAGCTCTACAGGAAGGATCCTCCGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGG
AGTTCTTCATCAAGCTTTTGAGCCACTGCCGAAAAAAATCGCCCCAGCGGTCACAGCCCGGTTTAAAATCATGGCCGATC
TCGATATTTCCGAACGTCGTCTTCCCCAGGATGGTAAAATTCGGCGGATGTATCAAGGACGAAAAGTAGATTTTCGGGTT
AATACCCTGCCCAGTCGCTACGGGGAAAAAGTCTGTTTAAGGATTCTCGATAACTCGGCTACCCAGTTGGGTTTAGATAA
ACTAATCACCGATCGAAATACTCTGCAAATTTTCCGGGACTTGGCCAGTCGTCCCTTTGGTCTGTTGCTGGTGACAGGCC
CCACTGGTTCGGGAAAATCCACCACTTTATACTCGGTGTTAGCGGAAAGAAATCACCCCGGAATTAATATTAATACCGCC
GAAGACCCGATCGAATATTCTCTGCCAGGCATCACCCAGGTGCAGGTAATTCGCGAAAAAGGCATGGATTTCGCCTCGAT
TCTGCGGGCATTCCTGCGACAAGATCCGGATGTGATTCTGGTGGGAGAAACTAGGGATAAGGAAACGGCTAAAACGGTGA
TCGAGGCCGCCTTAACCGGTCACTTGGTCTTAACTACCCTACACACTAACGATGCCGCAGGTGCGATCGTTCGTCTTGAT
AAAATGGGGATTGAACCATTTATGATTTCTGGTTCTCTGTTGGGAATCTTGGCCCAGCGTCTCGTGCGGCGCGTCTGTAC
CCAGTGTCGGGTCGCCTATCACCCTAGTCAAGAGGAATTAGCCCGTTTTGGTCTTTCCGCTGCCAATGAACAGGAAGTGA
CTTTTTATAAGGCTAATACCCTAACTATGGAGGAAATTCAACAAGCGCGAAAACAGGGCAATCTCTGCCTTAAGTGTGCT
GGCAGCGGTTACAAAGATCGCATCGGTGTCTATGAGGTAATGCAGAATAGTGAACGAATACAGCAGTTAATTAACCAAGG
GGCGACAACCGATCGCATTAAGGAGGCGGCCGTTGATGAGGGCATGGTGACACTCCTAGCCTATAGTTTAAATCTGGTGC
GCGAGGGTTACACCACCCTCGAAGAAGTAGAACGGGTGACACTGACCGATTCTGGTCTAGAAGCGGAATTAAAAGCCAAA
CGGAAAAGCGGTTTAATCTGTAGTGGTTGTCGCGCTGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGAGACC
GCGATTTAGTTATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-179)

Type II/IV secretion system protein (T2SSE)

(237-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

72.508

98.659

0.715