Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BTOYO_RS06290 Genome accession   NC_022781
Coordinates   1246809..1247243 (-) Length   144 a.a.
NCBI ID   WP_000811503.1    Uniprot ID   -
Organism   Bacillus toyonensis BCT-7112     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1241809..1252243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTOYO_RS06275 (Btoyo_1245) - 1242977..1243534 (+) 558 WP_000062055.1 PadR family transcriptional regulator -
  BTOYO_RS06280 (Btoyo_1246) metE 1244058..1246346 (+) 2289 WP_001007623.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BTOYO_RS06285 (Btoyo_1247) - 1246384..1246773 (-) 390 WP_000424018.1 competence protein ComJ -
  BTOYO_RS06290 (Btoyo_1248) nucA/comI 1246809..1247243 (-) 435 WP_000811503.1 DNA-entry nuclease Machinery gene
  BTOYO_RS06295 (Btoyo_1249) - 1247482..1248231 (+) 750 WP_000387494.1 DUF3967 domain-containing protein -
  BTOYO_RS06300 (Btoyo_1250) - 1248270..1249544 (-) 1275 WP_000420366.1 sensor histidine kinase -
  BTOYO_RS06305 (Btoyo_1251) - 1249856..1250056 (+) 201 WP_000929282.1 hypothetical protein -
  BTOYO_RS06310 (Btoyo_1252) - 1250175..1251338 (+) 1164 WP_000434560.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=63427 BTOYO_RS06290 WP_000811503.1 1246809..1247243(-) (nucA/comI) [Bacillus toyonensis BCT-7112]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAGDRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=63427 BTOYO_RS06290 WP_000811503.1 1246809..1247243(-) (nucA/comI) [Bacillus toyonensis BCT-7112]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACGAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATTGATCGTGGTGGTGCTGGAGATAGAAGGAAA
TTATCGTTAGCTCCATATCCATCAAAAAAAGGCTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGGGCGCATATTGAATATATAAGTCCAGCGGATAATCGCGGAGCAGGTTCTTGGGTAGGAAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(50-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549

Multiple sequence alignment