Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LRK53_RS05685 Genome accession   NZ_CP088923
Coordinates   1319645..1320682 (+) Length   345 a.a.
NCBI ID   WP_027493071.1    Uniprot ID   -
Organism   Rhodanobacter thiooxydans strain FW510-R12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1314645..1325682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRK53_RS05660 (LRK53_05660) - 1314885..1315709 (+) 825 WP_235642554.1 cobalamin-binding protein -
  LRK53_RS05665 (LRK53_05665) - 1315924..1317096 (-) 1173 WP_027491452.1 IS4 family transposase -
  LRK53_RS05670 (LRK53_05670) - 1317631..1317777 (+) 147 WP_235642555.1 hypothetical protein -
  LRK53_RS05675 (LRK53_05675) - 1317893..1318468 (-) 576 WP_027493072.1 YggT family protein -
  LRK53_RS05680 (LRK53_05680) proC 1318465..1319289 (-) 825 WP_235642556.1 pyrroline-5-carboxylate reductase -
  LRK53_RS05685 (LRK53_05685) pilT 1319645..1320682 (+) 1038 WP_027493071.1 type IV pilus twitching motility protein PilT Machinery gene
  LRK53_RS05690 (LRK53_05690) pilU 1320855..1322021 (+) 1167 WP_027493070.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LRK53_RS05695 (LRK53_05695) - 1322090..1322467 (-) 378 WP_235642557.1 BON domain-containing protein -
  LRK53_RS05700 (LRK53_05700) - 1322708..1324492 (-) 1785 WP_027493068.1 hypothetical protein -
  LRK53_RS05705 (LRK53_05705) - 1324593..1325519 (-) 927 WP_027493067.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38481.12 Da        Isoelectric Point: 6.6651

>NTDB_id=634011 LRK53_RS05685 WP_027493071.1 1319645..1320682(+) (pilT) [Rhodanobacter thiooxydans strain FW510-R12]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHSLIYDIMSDKQRRDYEEFYETDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGSPPIFKELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHMLTIED
PIEFVHTSQKCLVNQREIHRDTLGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATVHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVVSQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQFGMQTLDQCL
QDLVKRGLVTRQQAQAYAKNKDNFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=634011 LRK53_RS05685 WP_027493071.1 1319645..1320682(+) (pilT) [Rhodanobacter thiooxydans strain FW510-R12]
ATGGACATTGCCGAACTGCTTGCCTTCTCGGTGAAGAACAAGGCCTCGGACCTGCACTTGTCCGCCGGGCTGCCGCCGAT
GATCCGCGTCGACGGTGATGTCCGCCGCATCAACATCCCCGCGCTCGAGCACAAGCAGGTGCACTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAAGAATTCTACGAAACCGACTTCTCGTTCGAGATTCCCGGGCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCGTCCGAGGTGCTGACGCTGGA
GGATCTCGGCTCGCCGCCTATCTTCAAGGAACTGATCGAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACCACATCAACAAGAACGAATACGGGCATATGCTCACGATCGAAGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGGTCAACCAGCGCGAGATCCACCGCGACACGCTGGGCTTCAATGA
AGCGTTGCGTTCGGCGCTGCGCGAAGACCCGGACTACATCCTGGTCGGCGAGTTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAGACCGGCCACCTGGTGTTCGCCACCGTGCATACCAGCTCGGCGGCGAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAAAGCCTGCGCGCGGTGGTCTCGCAGTC
GCTGCTGAAGAAGGTCGGCGGCGGGCGCATCGCGGCGCACGAGATCATGGTTGGCATACCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGTCGATCCAGACCGGCCAGCAGTTCGGCATGCAGACGCTGGACCAGTGCCTG
CAGGATCTGGTCAAGCGCGGCCTGGTGACGCGCCAGCAGGCGCAGGCCTACGCCAAGAACAAGGACAATTTCAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

77.907

99.71

0.777
  pilT Acinetobacter nosocomialis M2

77.907

99.71

0.777
  pilT Acinetobacter baumannii D1279779

77.907

99.71

0.777
  pilT Acinetobacter baumannii strain A118

77.907

99.71

0.777
  pilT Acinetobacter baylyi ADP1

76.744

99.71

0.765
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.048

97.391

0.672
  pilT Vibrio cholerae strain A1552

69.048

97.391

0.672
  pilT Neisseria meningitidis 8013

63.768

100

0.638
  pilT Neisseria gonorrhoeae MS11

63.478

100

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.116

100

0.481
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4
  pilU Acinetobacter baylyi ADP1

38.905

100

0.391
  pilU Vibrio cholerae strain A1552

38.439

100

0.386